RT info:eu-repo/semantics/article T1 Delphinidin reduces glucose uptake in mice jejunal tissue and human intestinal cells lines through FFA1/GPR40 A1 Hidalgo, Jorge A1 Teuber, Stefanie A1 Morera, Francisco J. A1 Ojeda, Camila A1 Flores, Carlos A. A1 Hidalgo, María A. A1 Núñez Llorente, Lucía A1 Villalobos Jorge, Carlos A1 Burgos, Rafael A. K1 Delphinidin K1 Delfinidina K1 Anthocyanins K1 Antocianina K1 Glucose K1 Glucosa AB Anthocyanins are pigments with antihyperglycemic properties, and they are potential candidates for developing functional foods for the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism of these beneficial effects of anthocyanins are, however, hard to explain, given their very low bioavailability due to poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect of the anthocyanidin delphinidin over intestinal glucose absorption. We show the direct effects of delphinidin on the intestine using jejunum samples from RF/J mice, and the human intestinal cell lines HT-29, Caco-2, and NCM460. By the use of specific pharmacological antagonists, we determined that delphinidin inhibits glucose absorption in both mouse jejunum and a human enterocytic cell line in a FFA1-dependent manner. Delphinidin also affects the function of sodium-glucose cotransporter 1 (SGLT1). Intracellular signaling after FFA1 activation involved cAMP increase and cytosolic Ca2+ oscillations originated from intracellular Ca2+ stores and were followed by store-operated Ca2+ entry. Taken together, our results suggest a new GPR-40 mediated local mechanism of action for delphinidin over intestinal cells that may in part explain its antidiabetic effect. These findings are promising for the search for new prevention and pharmacological treatment strategies for DM2 management. PB MDPI SN 1422-0067 YR 2017 FD 2017 LK http://uvadoc.uva.es/handle/10324/45033 UL http://uvadoc.uva.es/handle/10324/45033 LA eng NO International Journal of Molecular Sciences, 2017, vol. 18, n. 4. 18 p. NO Producción Científica DS UVaDOC RD 22-nov-2024