RT info:eu-repo/semantics/article
T1 Delphinidin reduces glucose uptake in mice jejunal tissue and human intestinal cells lines through FFA1/GPR40
A1 Hidalgo, Jorge
A1 Teuber, Stefanie
A1 Morera, Francisco J.
A1 Ojeda, Camila
A1 Flores, Carlos A.
A1 Hidalgo, María A.
A1 Núñez Llorente, Lucía
A1 Villalobos Jorge, Carlos
A1 Burgos, Rafael A.
K1 Delphinidin
K1 Delfinidina
K1 Anthocyanins
K1 Antocianina
K1 Glucose
K1 Glucosa
AB Anthocyanins are pigments with antihyperglycemic properties, and they are potential candidates for developing functional foods for the therapy or prevention of Diabetes mellitus type 2 (DM2). The mechanism of these beneficial effects of anthocyanins are, however, hard to explain, given their very low bioavailability due to poor intestinal absorption. We propose that free fatty acid receptor 1 (FFA1, also named GPR40), is involved in an inhibitory effect of the anthocyanidin delphinidin over intestinal glucose absorption. We show the direct effects of delphinidin on the intestine using jejunum samples from RF/J mice, and the human intestinal cell lines HT-29, Caco-2, and NCM460. By the use of specific pharmacological antagonists, we determined that delphinidin inhibits glucose absorption in both mouse jejunum and a human enterocytic cell line in a FFA1-dependent manner. Delphinidin also affects the function of sodium-glucose cotransporter 1 (SGLT1). Intracellular signaling after FFA1 activation involved cAMP increase and cytosolic Ca2+ oscillations originated from intracellular Ca2+ stores and were followed by store-operated Ca2+ entry. Taken together, our results suggest a new GPR-40 mediated local mechanism of action for delphinidin over intestinal cells that may in part explain its antidiabetic effect. These findings are promising for the search for new prevention and pharmacological treatment strategies for DM2 management.
PB MDPI
SN 1422-0067
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/45033
UL http://uvadoc.uva.es/handle/10324/45033
LA eng
NO International Journal of Molecular Sciences, 2017, vol. 18, n. 4. 18 p.
NO Producción Científica
DS UVaDOC
RD 12-sep-2024