RT info:eu-repo/semantics/article
T1 Multifunctional cells in human pituitary adenomas: Implications for paradoxical secretion and tumorigenesis
A1 Senovilla González, Laura
A1 Núñez Llorente, Lucía
A1 Campos, José María de
A1 Luis Román, Daniel Antonio de
A1 Romero Bobillo, Enrique
A1 Sánchez, Ana
A1 García-Sancho Martín, Francisco Javier
A1 Villalobos Jorge, Carlos
K1 Multifunctional cells
K1 Células multifuncionales
K1 Pituitary Adenomas
K1 Adenomas pituitarios
K1 Tumorigenesis
K1 Tumorigénesis
AB Pituitary adenomas are very common in humans. They are of monoclonal origin, very heterogeneous, and produce frequently paradoxical secretion. The normal anterior pituitary (AP) contains some unorthodox multifunctional cells able to store more than one AP hormone (polyhormonal) and/or to express multiple hypothalamic-releasing hormone receptors (multiresponsive). Multifunctional AP cells seem to be involved in plasticity processes such as transdifferentiation or paradoxical secretion. Here, we have characterized the single-cell phenotypes of 15 human pituitary tumors, including prolactinomas, nonfunctioning adenomas, and adenomas from multiple endocrine neoplasia type I (MEN-I) and pituitary Cushing’s disease patients. Individual tumor cells were typed according to expression of AP hormones and hypothalamic-releasing hormone receptors by combination of calcium imaging and multiple sequential immunocytochemistry in the same cells. We found a large heterogeneity among the different tumors. In eight of the 15 tumors studied, more than 80% of the cells presented a multifunctional phenotype. This may explain the occurrence of paradoxical secretion. In addition, our results suggest that human pituitary adenomas might derive from multifunctional cells. This is consistent with the existence of a link between pituitary plasticity and tumorigenesis.
PB Oxford University Press
SN 1945-7197
YR 2004
FD 2004
LK http://uvadoc.uva.es/handle/10324/45071
UL http://uvadoc.uva.es/handle/10324/45071
LA eng
NO The Journal of Clinical Endocrinology & Metabolism, 2004, vol. 89, n. 9. p. 4545-4552
NO Producción Científica
DS UVaDOC
RD 23-nov-2024