RT info:eu-repo/semantics/article
T1 Mitochondrial [Ca2+] oscillations driven by local high [Ca2+] domains generated by spontaneous electric activity
A1 Villalobos Jorge, Carlos
A1 Núñez Llorente, Lucía
A1 Chamero, Pablo
A1 Alonso Alonso, María Teresa
A1 García-Sancho Martín, Francisco Javier
K1 Mitochondria
K1 Mitocondrias
K1 Calcium
K1 Calcio
K1 Cell activation
K1 Activación celular
AB Mitochondria take up calcium during cell activation thus shaping Ca2+ signaling and exocytosis. In turn, Ca2+ uptake by mitochondria increases respiration and ATP synthesis. Targeted aequorins are excellent Ca2+ probes for subcellular analysis, but single-cell imaging has proven difficult. Here we combine virus-based expression of targeted aequorins with photon-counting imaging to resolve dynamics of the cytosolic, mitochondrial, and nuclear Ca2+ signals at the single-cell level in anterior pituitary cells. These cells exhibit spontaneous electric activity and cytosolic Ca2+oscillations that are responsible for basal secretion of pituitary hormones and are modulated by hypophysiotrophic factors. Aequorin reported spontaneous [Ca2+] oscillations in all the three compartments, bulk cytosol, nucleus, and mitochondria. Interestingly, a fraction of mitochondria underwent much larger [Ca2+] oscillations, which were driven by local high [Ca2+] domains generated by the spontaneous electric activity. These oscillations were large enough to stimulate respiration, providing the basis for local tune-up of mitochondrial function by the Ca2+ signal.
PB Elsevier
SN 0021-9258
YR 2001
FD 2001
LK http://uvadoc.uva.es/handle/10324/45075
UL http://uvadoc.uva.es/handle/10324/45075
LA eng
NO Journal of Biological Chemistry, 2001, vol. 276, n. 43. p. 40293-40297
NO Producción Científica
DS UVaDOC
RD 06-ago-2024