RT info:eu-repo/semantics/article
T1 Rose bengal and green light versus riboflavin–UVA cross-linking: Corneal wound repair response
A1 Lorenzo Martín, Elvira
A1 Gallego Muñoz, Patricia
A1 Ibares Frías, Lucía
A1 Marcos, Susana
A1 Pérez Merino, Pablo
A1 Fernández Martínez, Itziar
A1 Kochevar, Irene E.
A1 Martínez García, María del Carmen
K1 Wound repair
K1 Cicatrización
K1 Cross-linking technique
K1 Técnica de cross-linking
K1 Rose bengal
K1 Rosa de Bengala
K1 Ultraviolet radiation
K1 Radiación ultravioleta
K1 Cornea
K1 Córnea
K1 2209.15 Optometría
AB Purpose: To study corneal wound healing after two cross-linking techniques using either rose bengal and green light (RGX) or the conventional treatment using riboflavin and UVA radiation (UVX). Methods: Corneas of New Zealand rabbits were monolaterally treated with UVX (21 eyes) or RGX (25 eyes). Treatments involved corneal de-epithelialization (8-mm diameter), soaking with photosensitizer (0.1% riboflavin in 20% dextran for 30 minutes for UVX; 0.1% rose bengal for 2 minutes for RGX), and light irradiation (370 nm, 3 mW/cm2, 30 minutes for UVX; 532 nm, 0.25 W/cm2, 7 minutes for RGX). Contralateral eyes were used as controls. Clinical follow-up included fluorescein staining, haze measurement, and pachymetry. Healing events analyzed after euthanasia at 2, 30, and 60 days included cell death (TUNEL assay), cell proliferation (BrdU [bromodeoxyuridine] immunofluorescence), and differentiation to myofibroblasts (α-SMA [alpha smooth muscle actin] immunohistochemistry). Results: Re-epithelialization and pachymetries were similar after RGX and UVX. The haze from day 1 to 15 was greater after UVX. Cell death was deeper after UVX, being localized in the anterior and middle stroma, and was superficial (anterior third) after RGX. Cell proliferation appeared after 2 days and was localized in the middle and posterior stroma in the UVX group but was superficial in the RGX group. After 60 days the number of stromal cells had not returned to the control number in either group. Conclusions: The deeper and longer-lasting cell damage caused by UVX compared to RGX may underlie the slower cell repopulation after UVX and other differences in healing. Shallower damage and a shorter treatment time suggest that RGX may be appropriate for stiffening thin corneas.
PB Association for Research in Vision and Ophthalmology
SN 1552-5783
YR 2018
FD 2018
LK http://uvadoc.uva.es/handle/10324/45095
UL http://uvadoc.uva.es/handle/10324/45095
LA eng
NO Investigative Ophthalmology & Visual Science, 2018, vol. 59. p. 4821-4830
NO Producción Científica
DS UVaDOC
RD 06-ago-2024