RT info:eu-repo/semantics/article
T1 Corneal wound repair after rose bengal and green light crosslinking: Clinical and histologic study
A1 Gallego Muñoz, Patricia
A1 Ibares Frías, Lucía
A1 Lorenzo Martín, Elvira
A1 Marcos, Susana
A1 Pérez Merino, Pablo
A1 Bekesi, Nandor
A1 Kochevar, Irene E.
A1 Martínez García, María del Carmen
K1 Wound repair
K1 Cicatrización
K1 Cross-linking technique
K1 Técnica de cross-linking
K1 Keratoconus treatment
K1 Tratamiento del queratocono
K1 Rose bengal
K1 Rosa de Bengala
K1 Cornea
K1 Córnea
K1 2209.15 Optometría
AB Purpose: To evaluate corneal wound healing after treatment with a new collagen crosslinking protocol using rose bengal dye and green light (RGX).Methods: One cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter circle and, in another group (n = 25), the DE corneas were then stained with 0.1% rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes (RGX). The contralateral eyes without treatment acted as controls. The animals were clinically followed including fluorescein staining and pachymetry. Healing events were analyzed after euthanasia at 2, 30, and 60 days. Cell death (TUNEL assay), cell proliferation (5-bromo-2′-deoxyuridine incorporation), and cell differentiation to myofibroblasts (α-SMA labeling) were carried out. In addition, loss of keratocytes and subsequent repopulation of the corneal stroma were quantified on hematoxylin-eosin–stained sections.Results: Wound closure was slower after RGX (4.4 days) then after DE (3.3 days). Cell death was restricted to the anterior central stroma, and the cellular decrease did not differ significantly between RGX and DE corneas. Cell proliferation in the epithelium and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the epithelium was complete at day 30, although cell repopulation of the stroma was not complete at 60 days.Conclusions: The healing response in corneas after RGX is very similar to that observed after DE alone, suggesting that, along with its short treatment time and limited effect on keratocytes, RGX displays good potential for clinical cornea stiffening.
PB Association for Research in Vision and Ophthalmology
SN 1552-5783
YR 2017
FD 2017
LK http://uvadoc.uva.es/handle/10324/45096
UL http://uvadoc.uva.es/handle/10324/45096
LA eng
NO Investigative Ophthalmology & Visual Science, 2017, vol. 58, n. 9. p. 3471-3480
NO Producción Científica
DS UVaDOC
RD 24-nov-2024