RT info:eu-repo/semantics/article
T1 Correlation between DNA synthesis in the second, third and fourth generations of spermatogonia and the occurrence of apoptosis in both spermatogonia and spermatocytes
A1 Blanco Rodríguez, Josefa
A1 Martínez García, María del Carmen
A1 Porras Gallo, María Almudena
K1 DNA synthesis
K1 Síntesis de ADN
K1 Spermatogonia
K1 Espermatogonia
K1 Apoptosis
K1 Apoptosis
K1 Spermatocytes
K1 Espermatocitos
AB In the seminiferous epithelium, both DNA synthesis andapoptosis occur at equivalent stages in various species, withapoptosis taking place mainly at the same stages as DNAreplication in the second, third and fourth spermatogonialgenerations. As preservation of the cellular associationsfound at these stages may have some functional significance, it is important to determine whether there is acorrelation between these cellular events. In this study,pairs of immunoperoxidase-stained adjacent testis sectionsfrom rats, mice, rabbits and cats in which either bromodeoxyuridine incorporated into the newly synthesizedDNA strand (BrdU labelling) or DNA 3  end labellingof the apoptotic DNA fragments (TUNEL assay) weredetected were compared. In addition, both events wereanalysed in double-labelled sections. These two methodsrevealed a clear correlation between the occurrence ofDNA replication in the second to fourth generations ofspermatogonia and most physiological apoptosis takingplace in both spermatogonia and spermatocytes in thethree different mammalian orders (Rodentia, Lagomorphaand Carnivora). This correlation may result from thesynchronization of mitotic spermatogonial and meioticspermatocyte cell cycle checkpoints operating at thesestages.
PB Bioscientifica
SN 1741-7899
YR 2003
FD 2003
LK http://uvadoc.uva.es/handle/10324/45116
UL http://uvadoc.uva.es/handle/10324/45116
LA eng
NO Reproduction, 2003, vol. 126, n. 5. p. 661-668
NO Producción Científica
DS UVaDOC
RD 08-ago-2024