RT info:eu-repo/semantics/article
T1 Apoptosis pattern elicited by oestradiol treatment of the seminiferous epithelium of the adult rat
A1 Blanco Rodríguez, Josefa
A1 Martínez García, María del Carmen
K1 Apoptosis
K1 Oestradiol treatment
K1 Tratamiento de estradiol
K1 Seminiferous epithelium
K1 Epitelio seminífero
AB It is widely assumed that oestrogen administration in the male mimics hypophysectomy by suppressing gonadotrophin secretion. Nevertheless, oestradiol treatment can increase germ-cell apoptosis mainly at stages IV–X of the spermatogenic cycle, rather than at stage VII when apoptotic germ-cell death is mainly triggered by gonadotrophin withdrawal caused by hypophysectomy. Since the roles of testicular oestrogens in spermatogenic regulation, if any, are unknown, we re-evaluated the germ-cell types that undergo apoptosis after oestradiol treatment. Adult male rats were injected daily with 50 μg oestradiol, oestradiol plus testosterone propionate (25 mg every 3 days) or oestradiol plus human menopausal gonadotrophin (equivalent to 25 iu FSH plus 25 iu LH) for 15 days. Apoptosis was assessed by in situ 3′-end labelling of internucleosomal DNA fragments in plastic semithin sections; the germ-cell types involved were identified by high-resolution light microscopy. The quantitative analysis of our results shows that the apoptosis pattern elicited by oestradiol treatment of the seminiferous epithelium differs from that reported to be caused by gonadotrophin or testosterone withdrawal, suggesting a possible role for oestradiol in the modulation of germ-cell death in the adult testis of the rat.
PB Bioscientifica
SN 1741-7899
YR 1997
FD 1997
LK http://uvadoc.uva.es/handle/10324/45118
UL http://uvadoc.uva.es/handle/10324/45118
LA eng
NO Journal of Reproduction and Fertility, 1997, vol. 110, n. 1. p. 61-70
NO Producción Científica
DS UVaDOC
RD 27-dic-2024