RT info:eu-repo/semantics/article
T1 Structuring diluted wheat matrices: impact of heat moisture treatment on protein aggregation and viscoelasticity of hydrated composite flours
A1 Collar Esteve, Concepción
A1 Villanueva Barrero, Marina
A1 Ronda Balbás, María Felicidad
K1 Teff
K1 Millet
K1 Chestnut
K1 Chickpea
K1 Protein aggregation
K1 Viscoelasticity
K1 3309 Tecnología de Los Alimentos
K1 3309.04 Panadería
AB The influence of heat-moisture treatment (HMT) and flour hydration (DY) on the restoration of dough viscoelasticity of wheat/non-wheat binary matrices was investigated by applying fundamental and empirical rheological procedures, and the protein structural reorganisation was monitored by measuring residual protein solubility in different media, and by assessing the accessibility of thiol groups inside the protein network. Single chestnut (CN), chickpea (CP), millet (MI) and teff (T) flour samples submitted to HMT (15% moisture content, 1 h and 120ºC) were blended with wheat flour at 10% (CN, CP) and 30% (MI, T) of replacement, and binary matrices hydrated at low (L), medium (M) and high (H) DY. Structuring ability of HMT was mainly observed in cereal flour blends (T, MI), where higher elastic moduli and lower loss tangent together with solid-like elastic structure over higher shear stress were observed as compared with treated non-cereal flour blends (CN. CP). Increased flour hydration significantly weakened blends structure, inducing a stepped decrease in dynamic moduli values particularly noticed in cereal blends at highest level of flour hydration, and a shift from elastic-like to viscous-like structure at lower shear stress in non-wheat cereal matrices. The formation of a protein network with reinforced compact structure associated to the presence or formation of intramolecular (CN, CP, T) and intermolecular disulphide bonds (CN, CP, MI, T), water soluble (CN, MI) and water insoluble aggregates (CP, T) is feasible to achieve with proteins of non-wheat flours submitted to HMT, particularly in high DY doughs. The lower the amount of free thiols in high molecular weight proteins encompassing high degree of crosslinking, corresponded to thermally treated samples (T, MI) blended at L and M hydration levels. For thermally treated samples, the lower the amount of free thiols in high molecular weight proteins encompassing high degree of crosslinking, corresponded to T and MI binary matrices blended at L and M hydration levels. These samples exhibited a solid-like elastic structure over higher shear stress and showed increased tolerance to stress/ strain before losing the structure.
PB Springer
SN 1935-5149
YR 2020
FD 2020
LK http://uvadoc.uva.es/handle/10324/45380
UL http://uvadoc.uva.es/handle/10324/45380
LA eng
NO Food and Bioprocess Technology, 2020, Volume 13, p. 475–487
NO Producción Científica
DS UVaDOC
RD 24-abr-2024