RT info:eu-repo/semantics/article
T1 Chondroitin sulphate mediated fusion of brain: neural folds in rat embryons
A1 Alonso Revuelta, María Isabel
A1 Moro Balbás, José Antonio
A1 Martín, C.
A1 Mano Bonín, Anibal de la
A1 Carnicero Gila, Estela María
A1 Martínez Álvarez, C.
A1 Navarro, N.
A1 Cordero, J.
A1 Gato Casado, Ángel Luis
K1 Tubo neural - obstrucción
K1 Embriología animal
AB Previous studies have demonstrated that during neural fold fusion in different species, an apical extracellular material rich in glycoconjugates is involved. However, the composi- tion and the biological role of this material remain undeter- mined. In this paper, we show that this extracellular matrix in rat increases notably prior to contact between the neural folds, suggesting the dynamic behaviour of the secretory process.  Immunostaining has allowed us to demonstrate that this extracellular matrix contains chondroitin sulphate proteoglycan (CSPG),  with a spatio-temporal distribution pattern, suggesting a direct relationship with the process of adhesion. The degree of CSPG involvement in cephalic neu- ral fold fusion in rat embryos was determined by treatment with specific glycosidases. In vitro rat embryo culture and microinjection techniques were employed to carry out se- lective digestion, with chondroitinase AC, of the CSPG on the apical surface of the neural folds; this was done immediately prior to the bonding of the cephalic neural folds. In all the treated embryos, cephalic defects of neural fold fusion could tant role in the fusion of the cephalic neural folds in rat em- bryos,  which implies that this proteoglycan could be in- volved in cellular recognition and adhesion.Abbreviations used in this paperCSPG                   chondroitin sulphate proteoglycanHSPG                   heparan sulphate proteoglycanPBS                      phosphate-buffered salineSEM                     scanning electron microscopy be detected. These results show that CSPG plays an impor-
PB Karger
SN 1422-6405
YR 2008
FD 2008
LK http://uvadoc.uva.es/handle/10324/4544
UL http://uvadoc.uva.es/handle/10324/4544
LA eng
NO Cells Tissues Organs, April 2008, n.2. p.1-16
NO Producción Científica
DS UVaDOC
RD 22-dic-2024