RT info:eu-repo/semantics/article
T1 TGF-β3-Induced Chondroitin Sulphate Proteoglycan
Mediates Palatal Shelf Adhesion
A1 Gato Casado, Ángel Luis
A1 Martínez, María Luisa
A1 Tudela, Consuelo
A1 Alonso Revuelta, María Isabel
A1 Moro Balbás, José Antonio
A1 Formoso, Miguel Ángel
A1 Ferguson, M. W. J.
K1 Neurología
K1 Embriología
AB TGF- 3-Induced Chondroitin Sulphate ProteoglycanMediates Palatal Shelf AdhesionA. Gato,* M. L. Martinez,† C. Tudela,† I. Alonso,* J. A. Moro,*M. A. Formoso,† M. W. J. Ferguson,‡ and C. Martı´nez-A´ lvarez†,1*Departamento de Anatomı´a Humana, Facultad de Medicina, Universidad de Valladolid,Valladolid 47005 Spain; †Departamento de Ciencias Morfolo´ gicas I, Facultad de Medicina,Universidad Complutense de Madrid, Madrid 28040, Spain; and ‡Cells, Immunology andDevelopment Division, School of Biological Sciences,University of Manchester, United KingdomIn mammals, the adhesion and fusion of the palatal shelves are essential mechanisms in the development of the secondarypalate. Failure of any of these processes leads to the formation of cleft palate. The mechanisms underlying palatal shelfadhesion are poorly understood, although the presence of filopodia on the apical surfaces of the superficial medial edgeepithelial (MEE) cells seems to play an important role in the adhesion of the opposing MEE. We demonstrate here theappearance of chondroitin sulphate proteoglycan (CSPG) on the apical surface of MEE cells only immediately prior tocontact between the palatal shelves. This apical CSPG has a functional role in palatal shelf adhesion, as either the alterationof CSPG synthesis by  -D-Xyloside or its specific digestion by chondroitinase AC strikingly alters the in vitro adhesion ofpalatal shelves. We also demonstrate the absence of this apical CSPG in the clefted palates of transforming growth factorbeta 3 (TGF- 3) null mutant mice, and its induction, together with palatal shelf adhesion, when TGF- 3 is added to TGF- 3null mutant palatal shelves in culture. When chick palatal shelves (that do not adhere in vivo nor express TGF- 3, nor CSPGin the MEE) are cultured in vitro, they do not express CSPG and partially adhere, but when TGF- 3 is added to the media,they express CSPG and their adhesion increases strikingly. We therefore conclude that the expression of CSPG on the apicalsurface of MEE cells is a key factor in palatal shelf adhesion and that this expression is regulated by TGF- 3. © 2002
PB Elsevier
SN 0012-1606/02
YR 2002
FD 2002
LK http://uvadoc.uva.es/handle/10324/4554
UL http://uvadoc.uva.es/handle/10324/4554
LA eng
NO Developmental Biology, 2002, vol.250. p.393-405
NO Producción Científica
DS UVaDOC
RD 20-nov-2024