RT info:eu-repo/semantics/article
T1 Quantification of IgM molecular response by droplet digital PCR as a potential tool for the early diagnosis of sepsis
A1 Tamayo Gómez, Eduardo
A1 Almansa Mora, Raquel
A1 Carrasco, Elena
A1 Ávila Alonso, Ana
A1 Rodríguez Fernández, Ana
A1 Wain, John
A1 Heredia Rodríguez, María
A1 Gómez Sánchez, Esther
A1 Soria, Susana
A1 Rico, Lucía
A1 Iglesias, Verónica
A1 Martínez Martínez, Ángel
A1 Andaluz Ojeda, David
A1 Gómez Herreras, José Ignacio
A1 Eiros Bouza, José María
A1 Bermejo Martín, Jesús Francisco
K1 Systemic inflammatory response
K1 Respuesta inflamatoria sistémica
K1 Molecular Response
K1 Respuesta molecular
K1 Abdominal Infection
K1 Infección abdominal
K1 Sepsis
AB Evaluation of host immune response to infection at the molecular level is a promising avenue to obtain diagnostic and prognostic tools for the clinical management of patients with sepsis. A recent report from Cajander and colleagues [1] has shown the potential of HLA-DR mRNA quantification by real-time PCR as a biomarker of immunosuppression in these patients. IgM is the first immunoglobulin produced in response to infection. In a pilot study, we have employed a next generation quantitative PCR method (nanoliter-sized droplet technology paired with digital PCR (ddPCR)) for detecting the early transcriptomic response of IgM in blood from patients with sepsis. Approval for the study protocol for both scientific and ethical aspects was obtained from the Committee for Clinical Research of Hospital Clínico Universitario, Valladolid, Spain. Written informed consent was obtained directly from each patient or a legal surrogate. The target gene transcript was IGHM, which encodes the constant region of the mu heavy chain, which defines the IgM isotype [2]. In blood, the cells producing IgM transcripts are B lymphocytes expressing CD20 [3], which was employed as housekeeping gene.
PB Springer Nature
SN 1364-8535
YR 2014
FD 2014
LK http://uvadoc.uva.es/handle/10324/45583
UL http://uvadoc.uva.es/handle/10324/45583
LA eng
NO Critical Care, 2014, vol. 18. 2 p.
NO Producción Científica
DS UVaDOC
RD 24-nov-2024