RT info:eu-repo/semantics/article
T1 Basal lamina heparan sulphate proteoglycan is involved in otic placode invagination in chick embryos
A1 Moro Balbás, José Antonio
A1 Gato Casado, Ángel Luis
A1 Alonso Revuelta, María Isabel
A1 Martín, P.
A1 Mano Bonín, Anibal de la
K1 Embriología
K1 Oido - Enfermedades
K1 Heparina
AB Invagination of other cup-shaped organ primordia. It is known that the cellular cytoskele- ton plays a Abstract  Formation of the otocyst from the otic placode appears to differ from limited role in otic placode invagination, whilst the extracellular matrix underlying the otic pri- mordium intervenes in the folding process. In this study we have analysed the role of the basal lamina heparan sulphate proteoglycan in otic primordium invagination. At  10  H.H.  stage,  heparan  sulphate  proteoglycan  im- the otic epithelium. Our findings support the theory that otic primordium invagination may be regulated, at least in part, by the basal lamina components, which might contribute towards anchoring the otic epithelium to adja- cent structures.Key words  Otic development • Heparinase • Microinjection • Epithelial folding • Extracellular matrix munomarking begins to appear on the otic placode basal	 	 lamina, increasing noticeably at 13 H.H. stage, coincid- ing with maximum folding of the otic epithelium, and is still present at later stages. Enzyme degradation of hepa- ran sulphate proteoglycan in the otic primordium basal lamina, by means of microinjection with heparinase III prior to folding, significantly disrupts invagination of the otic placode, which remains practically flat, with a sig- nificant reduction in the depth of the otic pit and an in- crease in the diameter of the otic opening. The immuno- cytochemistry analysis revealed a notable depletion of basal lamina heparan sulphate proteoglycan in the otic primordia microinjected with heparinase, with no statis- tically significant differences observed in the volume or rate of cell proliferation in the otic epithelium relative to the control, which suggests that heparan sulphate prot- eoglycan disruption does not interfere with the epithelial growth. In addition, a study of apoptosis distribution by the TUNEL method confirmed that treatment with hepa- rinase does not cause interference with cell survival in
PB Springer
SN 0340-2061
YR 2002
FD 2002
LK http://uvadoc.uva.es/handle/10324/4573
UL http://uvadoc.uva.es/handle/10324/4573
LA eng
NO Anat. Embryol., 2000, vol. 202. p. 333-343
NO Producción Científica
DS UVaDOC
RD 12-jul-2024