RT info:eu-repo/semantics/article
T1 Modeling of Polyhydroxyalkanoate Synthesis from Biogas byMethylocystis hirsuta
A1 Chen, Xueming
A1 Rodríguez Muñoz, Yadira
A1 López, Juan C.
A1 Muñoz Torre, Raúl
A1 Ni, Bing-Jie
A1 Sin, Gürkan
K1 Biogás
K1 Polihidroxialcanoato
K1 Biogas
K1 Polyhydroxyalkanoate
K1 23 Química
AB Methylocystis hirsuta, a type II methanotroph, has been experimentally demonstrated to be able to efficiently synthesize polyhydroxyalkanoates (PHA) from biogas under nutrient-limited conditions. A mechanistic model capable of describing the relevant processes of M. hirsuta, which is currently not available, would therefore lay a solid foundation for future practical demonstration and optimization of the PHA synthesis technology using biogas. To this end, dedicated batch tests were designed and conducted to obtain experimental data for different mechanistic processes of M. hirsuta. Through utilizing the experimental data of well-designed batch tests and following a step-wise model calibration/validation protocol, the stoichiometrics and kinetics of M. hirsuta are reported for the first time, including the yields of growth and PHA synthesis on CH4 (0.14 ± 0.01 g COD g–1 COD and 0.25 ± 0.02 g COD g–1 COD), the CH4 and O2 affinity constants (5.1 ± 2.1 g COD m–3 and 4.1 ± 1.7 g O2 m–3), the maximum PHA consumption rate (0.019 ± 0.001 g COD g–1 COD d–1), and the maximum PHA synthesis rate on CH4 (0.39 ± 0.05 g COD g–1 COD d–1). Through applying the developed model, an optimal O2:CH4 molar ratio of 1.6 mol O2 mol–1 CH4 was found to maximize the PHA synthesis by M. hirsuta. Practically, the model and parameters obtained would not only benefit the design and operation of bioreactors performing PHA synthesis from biogas, but also enable specific research on selection for type II methanotrophs in diverse environments.
PB American Chemical Society
SN 2168-0485
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/46748
UL https://uvadoc.uva.es/handle/10324/46748
LA eng
NO ACS Sustainable Chemistry & Engineering, 2020, vol. 8, n. 9, p. 3906-3912
NO Producción Científica
DS UVaDOC
RD 12-jul-2024