RT info:eu-repo/semantics/article
T1 Elucidating the influence of environmental factors on biogas-based polyhydroxybutyrate production by Methylocystis hirsuta CSC1
A1 Rodríguez Muñoz, Yadira
A1 Firmino, Paulo Igor Milen
A1 Arnáiz, Esther
A1 Lebrero Fernández, Raquel
A1 Muñoz Torre, Raúl
K1 Biogás
K1 Biogas
K1 Conversión de metano
K1 23 Química
AB The valorization of biogas as a feedstock for the generation of added-value bioproducts will play a key role on the sustainability of anaerobic digestion. The present work assessed the influence of key environmental parameters (O2:CH4 ratio, temperature and nitrogen source) on the growth and polyhydroxybutyrate (PHB) synthesis under nitrogen limiting conditions of the type II methanotroph Methylocystis hirsuta CSC1 using biogas as a feedstock. The O2:CH4 ratios tested (1:1, 1.5:1 and 2:1) did not affect significantly M. hirsuta CSC1 growth yields (~5 g TSS mol−1 CH4), although lower CH4 removal rates were reached under O2-limiting conditions (ratio 1:1). The highest PHB content (45 wt%) was achieved at a ratio 2:1 and was threefold higher than those obtained at lower ratios (~15 wt%). The increase in temperature from 15 to 25 °C resulted in increases in the growth yield (from 5 to 6 g TSS mol−1 CH4) and PHB content (from 32 to 40 wt%). Conversely, the lowest PHB content (30 wt%) was reached at 37 °C, together with a negligible growth under nutrient sufficient conditions. The nitrogen source also played a key role on both M. hirsuta CSC1 growth and PHB synthesis. Thus, ammonium resulted in the highest growth yield (7 g TSS mol−1 CH4), although the maximum PHB content was achieved when biomass was previously grown in nitrate as the nitrogen source (41 wt%). Nitrite exerted an inhibitory effect on M. hirsuta CSC1 growth.
PB Elsevier
SN 0048-9697
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/46751
UL https://uvadoc.uva.es/handle/10324/46751
LA eng
NO Science of The Total Environment, 2020, vol. 706, n. 1, p. 135136
NO Producción Científica
DS UVaDOC
RD 16-abr-2024