RT info:eu-repo/semantics/article
T1 High fat diet blunts the effects of leptin on ventilation and on carotid body activity
A1 Ribeiro, Maria J.
A1 Sacramento, Joana F.
A1 Gallego Martín, María Teresa
A1 Olea Fraile, Elena
A1 Melo, Bernardete F.
A1 Guarino, Maria P.
A1 Yubero Benito, Sara
A1 Obeso Cáceres, Ana María de la Luz
A1 Conde, Silvia V.
K1 Ventilation
K1 Ventilation
K1 Leptin
K1 Leptina
K1 Carotid body
K1 Cuerpo carotídeo
K1 Adenosine
K1 Adenosina
AB Leptin plays a role in the control of breathing, acting mainly on central nervous system structures. Leptin receptors are expressed in the carotid body (CB) and this finding has been associated with a putative physiological role of leptin in the regulation of CB function. Since, the CBs are implicated in energy metabolism, here we tested the effects of different concentrations of leptin administration on ventilatory parameters and on carotid sinus nerve (CSN) activity in control and high-fat (HF) diet fed rats, in order to clarify the role of leptin in ventilation control in metabolic disease states. We also investigated the expression of leptin receptors and the neurotransmitters involved in leptin signalling in the CBs. We found that in non-disease conditions, leptin increases minute ventilation in both basal and hypoxic conditions. However, in the HF model, the effect of leptin in ventilatory control is blunted. We also observed that HF rats display an increased frequency of CSN discharge in basal conditions that is not altered by leptin, in contrast to what is observed in control animals. Leptin did not modify intracellular Ca2+ in CB chemoreceptor cells, but it produced an increase in the release of adenosine from the whole CB. We conclude that CBs represent an important target for leptin signalling, not only to coordinate peripheral ventilatory chemoreflexive drive, but probably also to modulate metabolic variables. We also concluded that leptin signalling is mediated by adenosine release and that HF diets blunt leptin responses in the CB, compromising ventilatory adaptation.
PB The Physiological Society
SN 1469-7793
YR 2018
FD 2018
LK https://uvadoc.uva.es/handle/10324/46962
UL https://uvadoc.uva.es/handle/10324/46962
LA eng
NO The Journal of Physiology, 2018, vol. 596, n. 15. p. 3187-3199
NO Producción Científica
DS UVaDOC
RD 24-dic-2024