RT info:eu-repo/semantics/article
T1 Biocasting of an elastin-like recombinamer and collagen bi-layered model of the tunica adventitia and external elastic lamina of the vascular wall
A1 González Pérez, Miguel
A1 Camasão, Dimitria Bonizol
A1 Mantovani, Diego
A1 Alonso Rodrigo, Matilde
A1 Rodríguez Cabello, José Carlos
AB The development of techniques for fabricating vascular wall models will foster the development of preventiveand therapeutic therapies for treating cardiovascular diseases. However, the physical and biologicalcomplexity of vascular tissue represents a major challenge, especially for the design and the productionof off-the-shelf biomimetic vascular replicas. Herein, we report the development of a biocastingtechnique that can be used to replicate the tunica adventitia and the external elastic lamina of the vascularwall. Type I collagen embedded with neonatal human dermal fibroblast (HDFn) and an elastic click crosslinkable,cell-adhesive and protease-sensitive elastin-like recombinamer (ELR) hydrogel were investigatedas readily accessible and tunable layers to the envisaged model. Mechanical characterization confirmedthat the viscous and elastic attributes predominated in the collagen and ELR layers, respectively. In vitromaturation confirmed that the collagen and ELR provided a favorable environment for the HDFn viability,while histology revealed the wavy and homogenous morphology of the ELR and collagen layer respectively,the cell polarization towards the cell-attachment sites encoded on the ELR, and the enhancedexpression of glycosaminoglycan-rich extracellular matrix and differentiation of the embedded HDFn intomyofibroblasts. As a complementary assay, 30% by weight of the collagen layer was substituted with theELR. This model proved the possibility to tune the composition and confirm the versatile character of thetechnology developed, while revealing no significant differences with respect to the original construct.On-demand modification of the model dimensions, number and composition of the layers, as well as thetype and density of the seeded cells, can be further envisioned, thus suggesting that this bi-layered modelmay be a promising platform for the fabrication of biomimetic vascular wall models.
PB The Royal Society of Chemistry
SN 2047-4830
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/47857
UL https://uvadoc.uva.es/handle/10324/47857
LA spa
NO Biomater. Sci., 2021, 9, 3860–3874
NO Producción Científica
DS UVaDOC
RD 22-nov-2024