RT info:eu-repo/semantics/article
T1 Elucidating the key environmental parameters during the production of ectoines from biogas by mixed methanotrophic consortia
A1 Carmona Martínez, Alessandro A.
A1 Marcos Rodrigo, Eva
A1 Bordel Velasco, Sergio
A1 Marín de Jesús, David Fernando
A1 Herrero Lobo, Raquel
A1 García Encina, Pedro Antonio
A1 Muñoz Torre, Raúl
K1 Biogas
K1 Biogás
K1 Ectoine
K1 Ectoína
K1 Methanotrophs
K1 Metanotrofo
AB Anaerobic digestion (AD) is a robust biotechnology for the valorisation of organic waste into biogas. However, the rapid decrease in renewable electricity prices requires alternative uses of biogas. In this context, the engineering of innovative platforms for the bio-production of chemicals from CH4 has recently emerged. The extremolyte and osmoprotectant ectoine, with a market price of ~1000€/Kg, is the industrial flagship of CH4-based bio-chemicals. This work aimed at optimizing the accumulation of ectoines using mixed microbial consortia enriched from saline environments (a salt lagoon and a salt river) and activated sludge, and biogas as feedstock. The influence of NaCl (0, 3, 6, 9 and 12 %) and Na2WO4 (0, 35 and 70 μg L−1) concentrations and incubation temperature (15, 25 and 35 °C) on the stoichiometry and kinetics of the methanotrophic consortia was investigated. Consortia enriched from activated sludge at 15 °C accumulated the highest yields of ectoine and hydroxyectoine at 6 % NaCl (105.0 ± 27.2 and 24.2 ± 5.4 mgextremolyte gbiomass−1, respectively). The consortia enriched from the salt lagoon accumulated the highest yield of ectoine and hydroxyectoine at 9 % NaCl (56.6 ± 2.5 and 51.0 ± 2.0 mgextremolyte gbiomass−1, respectively) at 25 °C. The supplementation of tungsten to the cultivation medium did not impact on the accumulation of ectoines in any of the consortia. A molecular characterization of the enrichments revealed a relative abundance of ectoine-accumulating methanotrophs of 7–16 %, with Methylomicrobium buryatense and Methylomicrobium japanense as the main players in the bioconversion of methane into ectoine.
PB Elsevier
SN 0301-4797
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/48440
UL https://uvadoc.uva.es/handle/10324/48440
LA eng
NO Journal of Environmental Management, 2021, vol. 298, 113462
NO Producción Científica
DS UVaDOC
RD 01-may-2024