RT info:eu-repo/semantics/article
T1 Effective elastin-like recombinamers coating on poly(vinylidene) fluoride membranes for mesenchymal stem cell culture
A1 Guillot-Ferriols, Maria
A1 Barrio, Ana del
A1 Costa, Carlos M.
A1 Lanceros Méndez, Senentxu
A1 Rodríguez Cabello, José Carlos
A1 Gómez Ribelles, José Luis
A1 Santos, Mercedes
A1 Gallego Ferrer, Gloria
AB Bone’s inherent piezoelectricity is a key factor in regulating bone growth and mesenchymal stem cells (MSCs)fate towards the osteogenic lineage. The piezoelectric polymer poly(vinylidene) fluoride (PVDF) was thus used tomanufacture electroactive membranes by means of non-solvent induced phase separation (NIPS), producingporous membranes with approximately 90% of γ-phase for MSCs culture. The combination of the porous surfaceand PVDF hydrophobicity hinders cell adhesion and requires a coating to improve cell culture conditions. Alayer-by-layer (LbL) method was used to deposit elastin-like recombinamers (ELRs) containing RGD sequencesapplying click cross-linking chemistry. ELRs potential was confirmed by comparing traditional fibronectinadsorption with ELRs LbL on PVDF electroactive membranes. Porcine bone marrow MSCs preferred ELRs-coatedsurfaces, which enhanced initial cell adhesion and improved proliferation after 7 days. These findings lead tonew possibilities for regenerative therapies in the area of bone tissue engineering, offering the advantages of MSCcommitment towards the osteogenic lineage by applying electro-mechanical stimulation on electroactivesubstrates.
PB Elsevier
SN 0014-3057
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/48499
UL https://uvadoc.uva.es/handle/10324/48499
LA spa
NO European Polymer Journal 146 (2021) 110269 Available
NO Producción Científica
DS UVaDOC
RD 14-oct-2024