RT info:eu-repo/semantics/article
T1 Promoting laccase sensing activity for catechol detection using LBL assemblies of chitosan/ionic liquid/phthalocyanine as immobilization surfaces
A1 Salvo Comino, Coral
A1 García Hernández, Celia
A1 García Cabezón, Ana Cristina
A1 Rodríguez Méndez, María Luz
K1 Laccases
K1 Lacasas
K1 Biosensors
K1 Biosensores
K1 Catechol
K1 Catecol
K1 Phthalocyanine
K1 Ftalocianina
AB The performance of electrochemical laccase-based biosensors can be improved by immobilizing the enzyme on composite Layer-by-Layer (LbL) supports in which materials with complementary functions are combined. LbL films are formed by layers combining an electrocatalytic material which favors electron transfer (sulfonated copper phthalocyanine, CuPcS(−)), an ionic liquid which enhances the electrical conductivity of the layers (1-butyl-3-methylimidazolium tetrafluoroborate, IL(+)) and a material able to promote enzyme immobilization (chitosan, CHI(+)). Composite films with different structures have been demonstrated to be efficient electrocatalysts, producing an increase in the magnitude of the responses towards catechol. The most intense and reproducible electrocatalytic effect was observed when a layer of the CuPcS(−) was placed on top of a layer formed by a mixture of CHI(+) + IL(+) to obtain [CHI(+) + IL(+)|CuPcS(−)]2 films.Biosensors with laccase immobilized on the surface of the LbL layers [CHI(+) + IL(+)|CuPcS(−)]2|Lac showed mediated electron transfer between the redox enzyme and the film and a reproducibility of device-to-device performance of 4.1%. The amperometric biosensor showed a sensitivity of 0.237 A·M−1 and a linear detection range from 2.4 μM to 26 μM for catechol. The excellent Limit of detection (LOD) of 8.96·10−10 M (3·σ /m) is one order of magnitude lower than that obtained in similar studies. A Michaelis-Menten constant of 3.16 μM confirms excellent enzyme-substrate affinity.
PB Elsevier
SN 1567-5394
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/49112
UL https://uvadoc.uva.es/handle/10324/49112
LA eng
NO Bioelectrochemistry, 2020, vol. 132, 107407
NO Producción Científica
DS UVaDOC
RD 23-nov-2024