RT info:eu-repo/semantics/article
T1 FGF2 plays a key role in embryonic cerebrospinal fluid trophic properties over chick embryo neuroepithelial stem cells
A1 Martín, Cristina
A1 Bueno i Torrens, David
A1 Alonso Revuelta, María Isabel
A1 Moro Balbás, José Antonio
A1 Callejo, Sagrario
A1 Parada, Carolina
A1 Martín, P.
A1 Carnicero Gila, Estela María
A1 Gato Casado, Ángel Luis
K1 Tubo neural
K1 Células madre
K1 Neurogénesis
K1 Cerebro - Desarrollo
AB During early stages of brain development, neuroepithelial stem cells undergo intense proliferation as neurogenesis begins. Fibroblast growthfactor 2 (FGF2) has been involved in the regulation of these processes, and although it has been suggested that they work in an autocrine–paracrinemode, there is no general agreement on this because the behavior of neuroepithelial cells is not self-sufficient in explants cultured in vitro.In this work, we show that during early stages of development in chick embryos there is another source of FGF2, besides that of theneuroepithelium, which affects the brain primordium, since the cerebrospinal fluid (E-CSF) contains several isoforms of this factor. We alsodemonstrate, both in vitro and in vivo, that the FGF2 from the E-CSF has an effect on the regulation of neuroepithelial cell behavior, including cellproliferation and neurogenesis.In order to clarify putative sources of FGF2 in embryonic tissues, we detected by in situ hybridization high levels of mRNA expression innotochord, mesonephros and hepatic primordia, and low levels in brain neuroectoderm, corroborated by semiquantitative PCR analysis.Furthermore, we show that the notochord segregates several FGF2 isoforms which modify the behavior of the neuroepithelial cells in vitro. Inaddition, we show that the FGF2 ligand is present in the embryonic serum; and, by means of labeled FGF2, we prove that this factor passes via theneuroepithelium from the embryonic serum to the E-CSF in vivo.Considering all these results, we propose that, in chick embryos, the behavior of brain neuroepithelial stem cells at the earliest stages ofdevelopment is influenced by the action of the FGF2 contained within the E-CSF which could have an extraneural origin, thus suggesting a newand complementary way of regulating brain development.© 2006 Elsevier Inc. All rights reserved.
PB Elsevier
SN 0012-1606
YR 2006
FD 2006
LK http://uvadoc.uva.es/handle/10324/4947
UL http://uvadoc.uva.es/handle/10324/4947
LA eng
NO Developmental Biology, 2006, vol.297, p.402-416
NO Producción Científica
DS UVaDOC
RD 11-jul-2024