RT info:eu-repo/semantics/article
T1 Genetically engineered elastin-like recombinamers with sequence-based molecular stabilization as advanced bioinks for 3D bioprinting
A1 Salinas Fernández, Soraya
A1 Santos García, María Mercedes
A1 Alonso Rodrigo, Matilde
A1 Quintanilla Sierra, Luis
A1 Rodríguez Cabello, José Carlos
K1 Advanced materials
K1 Materiales avanzados
K1 Tissue engineering
K1 Ingeniería de tejidos
K1 Elastin like recombinamers
K1 Recombinantes tipo elastina
AB The versatility of 3D bioprinting techniques has demonstrated great potential for the development of artificial engineered tissues that more closely resemble native tissues. Despite this, challenges remain as regards the search for new bioinks that embrace all the complex parameters that this technique demands. In an attempt to develop such an advanced material, a novel smart material based on elastin-like recombinamers (ELRs) has been developed by molecularly programming its sequence to exhibit a sequential three-stage gelation process, thus providing printing attributes.The thermoresponsive behavior of the ELR is exploited for the deposition of well-controlled fibres on a platform heated to 37 °C, and its recombinant nature ensures batch-to-batch reproducibility and its applicability to a desired target tissue by the introduction of selected bioactive amino-acid sequences into its molecular chain.The biocompatible nature of the ELR enables the printing of loaded cells while providing a protective environment as part of the printing process. Thus, HFF-1 cells were found to be able to proliferate within the printed structures upon culture, displaying their natural morphology.The results of this work highlight the applicability and novelty of the bioprinting of biomimetic ELR-based structures for advanced applications.
PB Elsevier
SN 2352-9407
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/50974
UL https://uvadoc.uva.es/handle/10324/50974
LA eng
NO Applied Materials Today, 2020, vol. 18, 100500
NO Producción Científica
DS UVaDOC
RD 15-oct-2024