RT info:eu-repo/semantics/article
T1 The Contribution of Cytosolic Group IVA and Calcium-Independent Group VIA Phospholipase A2s to Adrenic Acid Mobilization in Murine Macrophages
A1 Monge, Patricia
A1 Garrido, Álvaro
A1 Rubio, Julio M.
A1 Magrioti, Victoria
A1 Kokotos, George
A1 Balboa García, María Ángeles
A1 Balsinde Rodríguez, Jesús
K1 Adrenic acid
K1 Ácido adrénico
K1 Arachidonic acid
K1 Ácido araquidónico
K1 Mass spectrometry
K1 Espectrometría de masas
K1 Macrophages
K1 Macrófagos
AB Adrenic acid (AA), the 2-carbon elongation product of arachidonic acid, is present at significant levels in membrane phospholipids of mouse peritoneal macrophages. Despite its abundance and structural similarity to arachidonic acid, very little is known about the molecular mechanisms governing adrenic acid mobilization in cells of the innate immune system. This contrasts with the wide availability of data on arachidonic acid mobilization. In this work, we used mass-spectrometry-based lipidomic procedures to define the profiles of macrophage phospholipids that contain adrenic acid and their behavior during receptor activation. We identified the phospholipid sources from which adrenic acid is mobilized, and compared the data with arachidonic acid mobilization. Taking advantage of the use of selective inhibitors, we also showed that cytosolic group IVA phospholipase A2 is involved in the release of both adrenic and arachidonic acids. Importantly, calcium independent group VIA phospholipase A2 spared arachidonate-containing phospholipids and hydrolyzed only those that contain adrenic acid. These results identify separate mechanisms for regulating the utilization of adrenic and arachidonic acids, and suggest that the two fatty acids may serve non-redundant functions in cells.
PB MDPI
SN 2218-273X
YR 2020
FD 2020
LK https://uvadoc.uva.es/handle/10324/52321
UL https://uvadoc.uva.es/handle/10324/52321
LA eng
NO Biomolecules 2020, vol. 10, n. 4, 542
NO Producción Científica
DS UVaDOC
RD 22-jun-2024