RT info:eu-repo/semantics/article T1 Optimization of nitrogen feeding strategies for improving polyhydroxybutyrate production from biogas by Methylocystis parvus OBBP in a stirred tank reactor A1 Rodríguez Muñoz, Yadira A1 García, Silvia María A1 Pérez Fernández, Rebeca A1 Lebrero Fernández, Raquel A1 Muñoz Torre, Raúl K1 Bioplastics K1 Bioplásticos K1 Biorefinery K1 Biorrefinería K1 Methylocystis parvus AB The design of efficient cultivation strategies to produce bioplastics from biogas is crucial for the implementation of this biorefinery process. In this work, biogas-based polyhydroxybutyrate (PHB) production and CH4 biodegradation performance was investigated for the first time in a stirred tank bioreactor inoculated with Methylocystis parvus OBBP. Decreasing nitrogen loading rates in continuous mode and alternating feast:famine regimes of 24 h-cycles, and alternating feast:famine regimes of 24 h:24 h and 24 h:48 h were tested. Continuous N feeding did not support an effective PHB production despite the occurrence of nitrogen limiting conditions. Feast-famine cycles of 24 h:24 h (with 50% stoichiometric nitrogen supply) supported the maximum PHB production (20 g-PHB m−3 d−1) without compromising the CH4-elimination capacity (25 g m−3 h−1) of the system. Feast:famine ratios ≤1:2 entailed the deterioration of process performance at stoichiometric nitrogen inputs ≤60%. PB Elsevier SN 0045-6535 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/52740 UL https://uvadoc.uva.es/handle/10324/52740 LA eng NO Chemosphere, 2022, vol. 299, 134443 NO Producción Científica DS UVaDOC RD 25-abr-2024