RT info:eu-repo/semantics/article
T1 Production of (R)-3-hydroxybutyric acid from methane by in vivo depolymerization of polyhydroxybutyrate in Methylocystis parvus OBBP
A1 Yáñez, Luz
A1 Rodríguez Muñoz, Yadira
A1 Scott, Felipe
A1 Vergara Fernández, Alberto
A1 Muñoz Torre, Raúl
K1 Polyhydroxybutyrate
K1 Polihidroxibutirato
K1 Chiral compounds
K1 Compuestos quirales
K1 Methanotrophic bacteria
K1 Metanotrofos
AB Methylocystis parvus OBBP accumulates polyhydroxybutyrate (PHB) using methane as the sole carbon and energy source. In this work, the feasibility of producing (R)-3-hydroxybutyric acid (R3HBA) via intracellularly accumulated PHB through depolymerization (in-vivo) was investigated. Results showed that a PHB to R3HBA conversion of 77.2 ± 0.9% (R3HBA titer of 0.153 ± 0.002 g L-1) can be attained in a mineral medium containing 1 g L-1 KNO3 at 30 °C with shaking at 200 rpm and a constant pH of 11 for 72 h. Nitrogen deprivation and neutral or acidic pHs strongly reduced the excreted R3HBA concentration. Reduced oxygen availability negatively affected the R3HBA yield, which decreased to 73.6 ± 4.9% (titer of 0.139 ± 0.01 g L-1) under microaerobic conditions. Likewise, the presence of increasing concentrations of R3HBA in the medium before the onset of PHB depolymerization reduced the initial R3HBA release rate and R3HBA yield.
PB Elsevier
SN 0960-8524
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/52834
UL https://uvadoc.uva.es/handle/10324/52834
LA eng
NO Bioresource Technology, 2022, vol. 353, 127141
NO Producción Científica
DS UVaDOC
RD 15-oct-2024