RT info:eu-repo/semantics/article
T1 Diurnal variation on tear stability and correlation with tear cytokine concentration
A1 Arroyo del Arroyo, Cristina
A1 Byambajav, Mungunshur
A1 Fernández Martínez, Itziar
A1 Martin, Eilidh
A1 González García, María Jesús
A1 López Miguel, Alberto
A1 Valencia Nieto, Laura
A1 Hagan, Suzanne
A1 Enriquez De Salamanca Aladro, Amalia
K1 Cytokines
K1 Citoquinas
K1 3201.09 Oftalmología
AB Purpose: To investigate the effect of time of day on tear evaporation rate (TER) and tear break-up time, and its possible relationship with the concentration of inflammatory tear molecules (cytokines) in healthy subjects. Methods: Participants with healthy ocular surfaces attended 3 visits, including the screening visit (V0), the 2nd visit (V1) and the 3rd visit (V2). There were 7-day intervals between visits. Participants with Dry Eye Disease (DED) were excluded by using appropriate clinical tests during V0. Clinical evaluation (TER and Non-Invasive Tear Break-Up Time (NITBUT)) and tear collection were performed during V1 and V2, between 9 and 10AM and 3-4PM. The relative humidity and temperature of the examination room were also measured. The tear fluid concentrations of 15 cytokines were measured by multiplex bead analysis. Results: Seven men and 10 women (mean age ± S.D; 25.1 ± 6.63 years old) participated in the study. There were no differences in neither the TER and NITBUT outcomes, nor humidity and temperature among times or visits. Eleven out of the 15 cytokines measured were detectable in tear fluids in > 50% of the participants. In the tear levels, no significant (p > 0.05) inter- and/or intra-day differences were detected for EGF, fractalkine, IL-1RA, IL-1β and IP-10. However, significant inter-day differences were found in the tear levels of IL-10 (p = 0.027), IFN-γ (p = 0.035) and TNF-α(p = 0.04) and intra-day differences in the tear levels of IL-8/CXCL8 (p = 0.034) and MCP-1 (p = 0.002). A significant correlation between TER and IL1-β, IL-2, and Fractalkine (p = 0.03, p = 0.03 and p = 0.046, respectively) was found at V1. Conclusions: NITBUT and TER values had no significant variability over the course of a day (AM versus PM), or on different days in healthy participants when humidity and temperature were constant. However, some tear molecule levels did show inter- and intra-day variability, having an inconsistent and moderate correlation with TER diurnal variation.
PB Elsevier
SN 1367-0484
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/53423
UL https://uvadoc.uva.es/handle/10324/53423
LA eng
NO Contact Lens and Anterior Eye, 2022, vol. 45, n. 6, 101705
NO Producción Científica
DS UVaDOC
RD 24-nov-2024