RT info:eu-repo/semantics/article T1 A cytochrome c encoded by thenaroperon is required for the synthesis of active respiratory nitrate reductase inThermus thermophilus A1 Zafra Amorós, Olga A1 Ramirez Arcos, Sandra A1 Castán García, Pablo A1 Moreno Albiger, Renata A1 Cava, Felipe A1 Valles Rapp, Cristina A1 Caro Azoy, Eddy A1 Berenguer Carlos, José K1 Biologia molecular K1 Bacterias K1 Respiración K1 Nitratos K1 Nitrate respiration K1 Thermophilic K1 Cytochrome c K1 Thermus AB A cytochrome c (NarC) is encoded as the first gene of the operon for nitrate respiration in Thermus thermophilus. NarC is required for anaerobic growth and for the synthesis of active nitrate reductase (NR). The α and δ subunits (NarG, NarJ) of the NR were constitutively expressed in narC::kat mutants, but NarG appeared in the soluble fraction instead of associated with the membranes. Our data demonstrate for NarC an essential role in the synthesis of active enzyme and for the attachment to the membrane of the respiratory NR from T. thermophilus. PB Federation of European Biochemical Societies SN 0014-5793 YR 2002 FD 2002 LK https://uvadoc.uva.es/handle/10324/53815 UL https://uvadoc.uva.es/handle/10324/53815 LA spa NO FEBS Letters, Vol. 523, Nº. 1-3, págs. 99-102 NO Producción Científica DS UVaDOC RD 22-nov-2024