RT info:eu-repo/semantics/article
T1 Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection
A1 Ares Sacristán, Ana María
A1 Martín Gómez, María Teresa
A1 Toribio Recio, Laura
A1 Bernal del Nozal, José
K1 Bee pollen
K1 Derivatization
K1 Fluorescence detection
K1 Free amino acid
K1 Liquid chromatography
K1 Solvent extraction
K1 23 Química
K1 2301 Química Analítica
AB Bee pollen is one of the hive products that is of most interest today due to its multiple beneficial health properties, making it an increasingly popular food supplement. Bee pollen contains many bioactive compounds, such as fatty acids, vitamins, minerals, proteins, and amino acids, among others. In the present study, the free amino acid content was determined in bee pollen by using liquid chromatography coupled to a fluorescence detector. Sample treatment consisted of a solvent extraction of the free amino acids with ultrapure water and a further centrifugation of the extract, which was repeated twice. After that, it was necessary to perform a pre-column derivatization of the amino acids using a combination of two reagents (o-phthalaldehyde and 9-fluorenylmethyl chloroformate) prior to their separation in a Gemini® C18 reverse phase column in gradient elution mode. The analytical performance was evaluated, and several commercial bee pollen samples were analyzed. Significant differences in the free amino acid profile and concentration, which ranged between 19 and 192 mg/g, were observed depending on the botanical origin of the samples.
PB Springer
SN 1936-9751
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/53850
UL https://uvadoc.uva.es/handle/10324/53850
LA eng
NO Food Analytical Methods,  volume 15, 2022, pages 2172–2180
NO Producción Científica
DS UVaDOC
RD 22-nov-2024