RT info:eu-repo/semantics/article
T1 Elastin-like hydrogel stimulates angiogenesis in a severe model of critical limb ischemia (CLI): An insight into the glyco-host response
A1 Marsico, Grazia
A1 Chunseng, Jin
AD 
A1 Abbah, Sunny A.
A1 Brauchle, Eva M.
A1 Thomas, Dilip
A1 Rebelo, Ana Lúcia
A1 Orbanic, Doriana
A1 Chantepie, Sandrine
A1 Contessotto, 
AD  Paolo
A1 Papy Garcia, Dulce
A1 Rodríguez Cabello, José Carlos
A1 Kilcoyne, Michelle
A1 Schenke, Layland
AD  K.
A1 Karlsson, N.G.
A1 McCullagh, Karl J.A.
A1 Pandit, Abhay
K1 Ischemia
K1 Glycosylation
K1 Angiogenesis
K1 24 Ciencias de la Vida
K1 22 Física
AB Critical limb ischemia (CLI) is characterized by the impairment of microcirculation, necrosis and inflammation of the muscular tissue. Although the role of glycans in mediating inflammation has been reported, changes in the glycosylation following muscle ischemia remains poorly understood.Here, a murine CLI model was used to show the increase of high mannose, α-(2, 6)-sialic acid and the decrease of hybrid and bisected N-glycans as glycosylation associated with the ischemic environment. Using this model, the efficacy of an elastin-like recombinamers (ELR) hydrogel was assessed. The hydrogel modulates key angiogenic signaling pathways, resulting in capillary formation, and ECM remodeling. Arterioles formation, reduction of fibrosis and anti-inflammatory macrophage polarization wa also induced by the hydrogel administration. Modulation of glycosylation was observed, suggesting, in particular, a role for mannosylation and sialylation in the mediation of tissue repair. Our study elucidates the angiogenic potential of the ELR hydrogel for CLI applications and identifies glycosylation alterations as potential new therapeutic targets.
PB Elsevier
SN 0142-9612
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/53893
UL https://uvadoc.uva.es/handle/10324/53893
LA eng
NO Biomaterials, 2021, vol. 269, p. 120641
NO Producción Científica
DS UVaDOC
RD 22-dic-2024