RT info:eu-repo/semantics/article T1 An interfacial self-assembling bioink for the manufacturing of capillary-like structures with tuneable and anisotropic permeability A1 Wu, Yuanhao A1 Fortunato, Gabriele Maria A1 Okesola, Babatunde O A1 Pellerej Di Brocchetti, Francesco Luigi A1 Suntornnond, Ratima A1 Connelly, John A1 De Maria, Carmelo A1 Rodríguez Cabello, José Carlos A1 Vozzi, Giovanni A1 Wang, Wen A1 Mata, Álvaro K1 Bioink K1 Biotinta K1 Self-assembling K1 Autoensamblaje K1 Anisotropic K1 Anisótropo K1 22 Física AB Self-assembling bioinks offer the possibility to biofabricate with molecular precision, hierarchical control, and biofunctionality. For this to become a reality with widespread impact, it is essential to engineer these ink systems ensuring reproducibility and providing suitable standardization. We have reported a self-assembling bioink based on disorder-to-order transitions of an elastin-like recombinamer (ELR) to co-assemble with graphene oxide (GO). Here, we establish reproducible processes, optimize printing parameters for its use as a bioink, describe new advantages that the self-assembling bioink can provide, and demonstrate how to fabricate novel structures with physiological relevance. We fabricate capillary-like structures with resolutions down to ∼10 µm in diameter and ∼2 µm thick tube walls and use both experimental and finite element analysis to characterize the printing conditions, underlying interfacial diffusion-reaction mechanism of assembly, printing fidelity, and material porosity and permeability. We demonstrate the capacity to modulate the pore size and tune the permeability of the resulting structures with and without human umbilical vascular endothelial cells. Finally, the potential of the ELR-GO bioink to enable supramolecular fabrication of biomimetic structures was demonstrated by printing tubes exhibiting walls with progressively different structure and permeability. PB IOP Publishing SN 1758-5082 YR 2021 FD 2021 LK https://uvadoc.uva.es/handle/10324/53898 UL https://uvadoc.uva.es/handle/10324/53898 LA eng NO Biofabrication, 2021, vol.13, n. 3, p. 035027 NO Producción Científica DS UVaDOC RD 10-dic-2024