RT info:eu-repo/semantics/article
T1 An interfacial self-assembling bioink for the manufacturing of capillary-like structures with tuneable and anisotropic permeability
A1 Wu, Yuanhao
A1 Fortunato, Gabriele Maria
A1 Okesola, Babatunde O
A1 Pellerej Di Brocchetti, Francesco Luigi
A1 Suntornnond, Ratima
A1 Connelly, John
A1 De Maria, Carmelo
A1 Rodríguez Cabello, José Carlos
A1 Vozzi, Giovanni
A1 Wang, Wen
A1 Mata, Álvaro
K1 Bioink
K1 Biotinta
K1 Self-assembling
K1 Autoensamblaje
K1 Anisotropic
K1 Anisótropo
K1 22 Física
AB Self-assembling bioinks offer the possibility to biofabricate with molecular precision, hierarchical control, and biofunctionality. For this to become a reality with widespread impact, it is essential to engineer these ink systems ensuring reproducibility and providing suitable standardization. We have reported a self-assembling bioink based on disorder-to-order transitions of an elastin-like recombinamer (ELR) to co-assemble with graphene oxide (GO). Here, we establish reproducible processes, optimize printing parameters for its use as a bioink, describe new advantages that the self-assembling bioink can provide, and demonstrate how to fabricate novel structures with physiological relevance. We fabricate capillary-like structures with resolutions down to ∼10 µm in diameter and ∼2 µm thick tube walls and use both experimental and finite element analysis to characterize the printing conditions, underlying interfacial diffusion-reaction mechanism of assembly, printing fidelity, and material porosity and permeability. We demonstrate the capacity to modulate the pore size and tune the permeability of the resulting structures with and without human umbilical vascular endothelial cells. Finally, the potential of the ELR-GO bioink to enable supramolecular fabrication of biomimetic structures was demonstrated by printing tubes exhibiting walls with progressively different structure and permeability.
PB IOP Publishing
SN 1758-5082
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/53898
UL https://uvadoc.uva.es/handle/10324/53898
LA eng
NO Biofabrication, 2021, vol.13, n. 3, p. 035027
NO Producción Científica
DS UVaDOC
RD 22-dic-2024