RT info:eu-repo/semantics/article
T1 The Effects of crosslinking on the rheology and cellular behavior of polymer-based 3D-multilayered scaffolds for restoring articular cartilage
A1 Campos, Yaima
A1 Sola, Francisco J.
A1 Fuentes, Gastón
A1 Quintanilla Sierra, Luis
A1 Almirall, Amisel
A1 Cruz, Luis J
A1 Rodríguez Cabello, José Carlos
A1 Tabata, Yasuhiko
K1 Multilayer scaffold
K1 Rheology
K1 Crosslinking
K1 22 Física
K1 23 Química
AB Polymer-based tri-layered (bone, intermediate and top layers) scaffolds used for the restoration of articular cartilage were prepared and characterized in this study to emulate the concentration gradient of cartilage. The scaffolds were physically or chemically crosslinked. In order to obtain adequate scaffolds for the intended application, the impact of the type of calcium phosphate used in the bone layer, the polymer used in the intermediate layer and the interlayer crosslinking process were analyzed. The correlation among SEM micrographs, physical-chemical characterization, swelling behavior, rheological measurements and cell studies were examined. Storage moduli at 1 Hz were 0.3–1.7 kPa for physically crosslinked scaffolds, and 4–5 kPa (EDC/NHS system) and 15–20 kPa (glutaraldehyde) for chemically crosslinked scaffolds. Intrinsic viscoelasticity and poroelasticity were considered in discussing the physical mechanism dominating in different time/frequency scales. Cell evaluation showed that all samples are available as alternatives to repair and/or substitute cartilage in articular osteoarthritis.
PB MDPI
SN 2073-4360
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/53933
UL https://uvadoc.uva.es/handle/10324/53933
LA eng
NO Polymers, 2021, vol. 13, n. 6, p. 907
NO Producción Científica
DS UVaDOC
RD 24-dic-2024