RT info:eu-repo/semantics/article
T1 Spatially heterogeneous tubular scaffolds for In situ heart valve tissue engineering using melt electrowriting
A1 Toosi Saidy, Navid
A1 Fernández Colino, Alicia
A1 Shiroud Heidari, Behzad
A1 Kent, Ross
A1 Vernon, Michael
A1 Bas, Onur
A1 Mulderrig, Shane
A1 Lubig, Andreas
A1 Rodríguez Cabello, José Carlos
A1 Doyle, Barry
A1 Hutmacher, Dietmar W.
A1 Juan Pardo, Elena M. de
A1 Mela, Petra
K1 Tubular scaffolds
K1 Tissue engineering
K1 Electrowriting
K1 22 Física
K1 24 Ciencias de la Vida
K1 32 Ciencias Médicas
AB Heart valve tissue engineering (HVTE) aims to provide living autologous heart valve implants endowed with regenerative capabilities and life-long durability. However, fabrication of biomimetic scaffolds capable of providing the required functionality in terms of mechanical performance and tunable porosity to enable cellular infiltration remains a major challenge. Here, the additive manufacturing of bioinspired, spatially heterogeneous, tubular scaffolds enclosing the leaflets, inter-leaflet triangles, and their interface for in situ HVTE using melt electrowriting (MEW) is demonstrated. The innovative platform enables the digital fabrication of scaffolds with ad hoc architecture (e.g., tunable location, specific fiber pattern, and orientation) and customizable geometry via a custom-made control software. The user-friendly interface allows for the definition of areas of the scaffold with specific patterns to obtain properties such as tunable J-shaped stress–stain curve and anisotropy typical of the heart valve leaflet, compliant inter-leaflet triangles, and reinforced curvilinear boundary between them. Heterogeneous, tubular, heart valve MEW scaffolds are then embedded with a microporous elastin-like recombinamer (ELR) hydrogel to develop a soft-network composite favoring cell infiltration and ensuring hemocompatibility. The acute systolic hemodynamic functionality of the MEW/ELR composite satisfies the ISO 5840 requirements, under aortic and pulmonary conditions.
PB Wiley
SN 1616-301X
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/54088
UL https://uvadoc.uva.es/handle/10324/54088
LA eng
NO Advanced Functional Materials, 2022, vol. 32, n. 21
NO Producción Científica
DS UVaDOC
RD 19-oct-2024