RT info:eu-repo/semantics/article
T1 Mineralizing coating on 3D printed scaffolds for the promotion of osseointegration
A1 Hasan, Abshar
A1 Bagnol, Romain
A1 Owen, Robert
A1 Latif, Arsalan
A1 Rostam, Hassan M.
A1 Elsharkawy, Sherif
A1 Rose, Felicity R. A. J.
A1 Rodríguez Cabello, José Carlos
A1 Ghaemmaghami, Amir M.
A1 Eglin, David
A1 Mata, Álvaro
K1 Cirugía maxilofacial
K1 Biomineralization
K1 Bone regeneration
K1 Regeneración ósea
K1 3D printing
K1 Impresión 3D
K1 2407 Biología Celular
K1 3213.13 Ortodoncia-Estomatología
AB Design and fabrication of implants that can perform better than autologous bone graftsremain an unmet challenge for the hard tissue regeneration in craniomaxillofacialapplications. Here, we report an integrated approach combining additivemanufacturing with supramolecular chemistry to develop acellular mineralizing 3Dprinted scaffolds for hard tissue regeneration. Our approach relies on an elastin-likerecombinamer (ELR) coating designed to trigger and guide the growth of ordered apatiteon the surface of 3D printed nylon scaffolds. Three test samples including a) uncoatednylon scaffolds (referred to as “Uncoated”), b) ELR coated scaffolds (referred to as “ELRonly”), and c) ELR coated and in vitro mineralized scaffolds (referred to as “Pre-mineralized”) were prepared and tested for in vitro and in vivo performance. All testsamples supported normal human immortalized mesenchymal stem cell adhesion,growth, and differentiation with enhanced cell proliferation observed in the “Pre-mineralized” samples. Using a rabbit calvarial in vivo model, ‘Pre-mineralized’ scaffoldsalso exhibited higher bone ingrowth into scaffold pores and cavities with higher tissue-implant integration. However, the coated scaffolds (“ELR only” and “Pre-mineralized”) didnot exhibit significantly more new bone formation compared to “Uncoated” scaffolds.Overall, the mineralizing coating offers an opportunity to enhance integration of 3D printedbone implants. However, there is a need to further decipher and tune their immunologicresponse to develop truly osteoinductive/conductive surfaces.
PB Frontiers Media
SN 2296-4185
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/55063
UL https://uvadoc.uva.es/handle/10324/55063
LA eng
NO Frontiers in Bioengineering and Biotechnology, 2022, vol. 10, artículo 836386
NO Producción Científica
DS UVaDOC
RD 12-sep-2024