RT info:eu-repo/semantics/article
T1 Effect of mTORC1/mTORC2 inhibition on T cell function: potential role in graft-versus-host disease control
A1 Herrero Sánchez, María del Carmen
A1 Rodríguez Serrano, Concepción
A1 Almeida Parra, Julia
A1 San Segundo Payo, Laura
A1 Inogés Sancho, Laura
A1 Santos-Briz Terrón, Ángel
A1 García Briñón, Jesús
A1 San Miguel Izquierdo, Jesús F.
A1 Cañizo Fernández-Roldán, Consuelo del
A1 Blanco Durango, Belén
K1 Graft versus host disease
K1 Cell transplantation
K1 Trasplante de células
K1 Pharmacology
K1 MTOR inhibitors
K1 3205.04 Hematología
K1 3201.01 Oncología
AB The mechanistic target of rapamycin (mTOR) pathway is crucial for the activation and function of T cells, which play an essential role in the development of graft-versus-host disease (GvHD). Despite its partial ability to block mTOR pathway, the mTORC1 inhibitor rapamycin has shown encouraging results in the control of GvHD. Therefore, we considered that simultaneous targeting of both mTORC1 and mTORC2 complexes could exert a more potent inhibition of T cell activation and, thus, could have utility in GvHD control. To assess this assumption, we have used the dual mTORC1/mTORC2 inhibitors CC214-1 and CC214-2. In vitro studies confirmed the superior ability of CC214-1 versus rapamycin to block mTORC1and mTORC2 activity and to reduce T cell proliferation. Both drugs induced a similar decrease in Th1/Th2 cytokine secretion, but CC214-1 was more efficient in inhibiting na€ıve T cell activation and the expression of Tcell activation markers. In addition, CC214-1 induced specific tolerance against alloantigens, while preserving anti-cytomegalovirus response. Finally, in a mouse model of GvHD, the administration of CC214-2 significantly improved mice survival and decreased GvHD-induced damages. In conclusion, the current study shows, for the first time, the immunosuppressive ability of CC214-1 on T lymphocytes and illustrates the role of CC214-2 in the allogeneic transplantation setting as a possible GvHD prophylaxis agent.
PB John Wiley & Sons
SN 0007-1048
YR 2016
FD 2016
LK https://uvadoc.uva.es/handle/10324/55160
UL https://uvadoc.uva.es/handle/10324/55160
LA eng
NO British Journal of Haematology (BJHaem), 2016, Vol. 173, Nº. 5, págs. 754–768
NO Producción Científica
DS UVaDOC
RD 22-dic-2024