RT info:eu-repo/semantics/article
T1 Prenatal expression of interleukin 1B and interleukin 6 in the rat pituitary gland
A1 Moro Balbás, José Antonio
A1 Carretero, José
A1 Alonso Revuelta, María Isabel
A1 Martín, C.
A1 Gato Casado, Ángel Luis
A1 Mano Bonín, Anibal de la
K1 Citoquímica
K1 Hipófisis - Cáncer
AB It is known that interleukin 1b (IL-1b) and interleukin 6 (IL-6) are expressed post-natally in normal andtumoral cells in the anterior pituitary, and that they play a role in both the liberation of different hormonesand in the growth, proliferation and tumor formation of the pituitary gland. However, theirexpression and role during embryonic and fetal development remain unknown. We have performed animmunocytochemistry study of prenatal expression and distribution of IL-1b and IL-6 in isolated embryonicrat Rathke’s pouch prior to birth, more specifically between 13.5 and 19.5 days p.c. Western-blotanalysis carried out on 19.5-day p.c. embryos showed positive immunolabelling for IL-1b and IL-6. Theseinterleukins were initially expressed simultaneously in the rostral and ventral portions of Rathke’s pouchin 15.5-day p.c. embryos, and this expression progressed caudodorsally in later developmental stages,extending to most of the hypophysis before birth. The number of cells expressing these interleukinsincreased throughout this period: 48.22% of anterior pituitary cells expressed IL-6 in 19.5-day embryos,whilst IL-1b was positive in 39.8% of the cells. Moreover, we have demonstrated that some adenohypophysealcells co-express both interleukins. Such findings represent the first step towards an understandingof the physiological role of these interleukins in anterior pituitary development.
PB Elsevier
YR 2008
FD 2008
LK http://uvadoc.uva.es/handle/10324/5540
UL http://uvadoc.uva.es/handle/10324/5540
LA eng
NO Citokine, XXX, 2008
NO Producción Científica
DS UVaDOC
RD 26-dic-2024