RT info:eu-repo/semantics/article
T1 Olive pomace phenolic compounds stability and safety evaluation: from raw material to future ophthalmic applications
A1 Katsinas, Nicolaos
A1 Enriquez De Salamanca Aladro, Amalia
A1 Bento da Silva, Andreia
A1 Bronze, Maria Rosario
A1 Rodríguez Rojo, Soraya
K1 Ojo - Enfermedades y defectos - Tratamiento
K1 Aceite de oliva
K1 Aceite de oliva -  Análisis
K1 Olive pomace
K1 Phenolic extracts
K1 3201.09 Oftalmología
AB Nowadays, increasing interest in olive pomace (OP) valorization aims to improve olive’s industry sustainability. Interestingly, several studies propose a high-value application for OP extracts containing its main phenolic compounds, hydroxytyrosol and oleuropein, as therapy for ocular surface diseases. In this work, the stability and accessibility of OP total phenolic and flavonoid content, main representative compounds, and antioxidant activity were assessed under different pretreatment conditions. Among them, lyophilization and supercritical CO2 extraction were found to increase significantly most responses measured in the produced extracts. Two selected extracts (CONV and OPT3) were obtained by different techniques (conventional and pressurized liquid extraction); Their aqueous solutions were characterized by HPLC-DAD-MS/MS. Additionally, their safety and stability were evaluated according to EMA requirements towards their approval as ophthalmic products: their genotoxic effect on ocular surface cells and their 6-months storage stability at 4 different temperature/moisture conditions (CPMP/ICH/2736/99), together with pure hydroxytyrosol and oleuropein solutions. The concentration of hydroxytyrosol and oleuropein in pure or extract solutions was tracked, and possible degradation products were putatively identified by HPLC-DAD-MS/MS. Hydroxytyrosol and oleuropein had different stability as standard or extract solutions, with oleuropein also showing different degradation profile. All compounds/extracts were safe for ophthalmic use at the concentrations tested.
PB MDPI
SN 1420-3049
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/55611
UL https://uvadoc.uva.es/handle/10324/55611
LA eng
NO Molecules, 2021,  Vol. 26, Nº. 19, 23 pp.
NO Producción Científica
DS UVaDOC
RD 24-nov-2024