RT info:eu-repo/semantics/article
T1 Olive pomace phenolic compounds and extracts can inhibit inflammatory- and oxidative-related diseases of human ocular surface epithelium
A1 Katsinas, Nicolaos
A1 Rodríguez Rojo, Soraya
A1 Enriquez De Salamanca Aladro, Amalia
K1 Cornea - Diseases
K1 Conjunctiva - Diseases
K1 Ojo - Enfermedades y defectos - Tratamiento
K1 Ojo seco
K1 Aceite de oliva
K1 Aceite de oliva -  Análisis
K1 Antioxidantes
K1 Olive pomace
K1 Phenolic extracts
K1 3201.09 Oftalmología
AB Oxidative- and inflammatory-related ocular surface diseases have high prevalence and are an emerging issue in ophthalmology. Olive pomace (OP) is the olive oil’s industry main by-product, and is potentially environmentally hazardous. Nevertheless, it contains phenolic compounds with important bioactivities, like oleuropein (OL) and hydroxytyrosol (HT). The antioxidant and anti-inflammatory effects of four OP extracts (CONV, OPT(1–3)), pure OL and HT, and mixtures thereof were screened on human corneal (HCE) and conjunctival epithelial (IM-ConjEpi) cells. CONV was conventionally extracted, while OPT(1–3) were produced by pressurized liquid extraction. Thanks to their improved activity, CONV and OPT3 (HT-enriched) were selected for dose-dependent studies. Cells were stimulated with tumor necrosis factor-α or ultraviolet-B radiation, measuring interleukin (IL)-1β, IL-6, IL-8, and IL-17A as well as interferon γ-induced protein [IP]-10 secretion or intracellular ROS production, respectively. On HCE, both extracts and HT inhibited the secretion of most measured ILs, demonstrating a strong anti-inflammatory effect; while in IM-ConjEpi, all samples decreased IP-10 secretion. Moreover, HT, OL, and both extracts showed strong dose-dependent antioxidant activity in both cell lines. Compared with CONV, OPT3 was active at lower concentrations, demonstrating that intensified extraction techniques are selective towards targeted biomarkers. Hence, a high-value application as potential ocular surface therapy was proposed for the OP valorization.
PB MDPI
SN 2076-3921
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/55613
UL https://uvadoc.uva.es/handle/10324/55613
LA eng
NO Antioxidants, 2021, Vol. 10, Nº. 7, 25 pp.
NO Producción Científica
DS UVaDOC
RD 19-abr-2024