RT info:eu-repo/semantics/article T1 Influence of key operational parameters on biohydrogen production from fruit and vegetable waste via lactate-driven dark fermentation A1 Martínez Mendoza, Leonardo José A1 Lebrero Fernández, Raquel A1 Muñoz Torre, Raúl A1 García Depraect, Octavio K1 Dark fermentation K1 Fermentación oscura K1 Hydrogen production K1 Hidrógeno - Producción AB This study aims at investigating the influence of operational parameters on biohydrogen production from fruit-vegetable waste (FVW) via lactate-driven dark fermentation. Mesophilic batch fermentations were conducted at different pH (5.5, 6.0, 6.5, 7.0, and non-controlled), total solids (TS) contents (5, 7, and 9%) and initial cell biomass concentrations (18, 180, and 1800 mg VSS/L). Higher hydrogen yields and rates were attained with more neutral pH values and low TS concentrations, whereas higher biomass densities enabled higher production rates and avoided wide variations in hydrogen production. A marked lactate accumulation (still at neutral pH) in the fermentation broth was closely associated with hydrogen inhibition. In contrast, enhanced hydrogen productions matched with much lower lactate accumulations (even it was negligible in some fermentations) along with the acetate and butyrate co-production but not with carbohydrates removal. At pH 7, 5% TS, and 1800 mg VSS/L, 49.5 NmL-H2/g VSfed and 976.4 NmL-H2/L-h were attained. PB Elsevier SN 0960-8524 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/55778 UL https://uvadoc.uva.es/handle/10324/55778 LA eng NO Bioresource Technology, 2022, vol. 364, 128070 NO Producción Científica DS UVaDOC RD 26-dic-2024