RT info:eu-repo/semantics/article
T1 Cellular plasmalogen content does not influence arachidonic acid levels or distribution in macrophages: A role for cytosolic phospholipase A2γ in phospholipid remodeling
A1 Lebrero Fernández, Patricia
A1 Astudillo, Alma M.
A1 Astudillo del Valle, Alma María
A1 Rubio, Julio M.
A1 Fernández-Caballero Palomeque, Lidia
A1 Kokotos, George
A1 Balboa García, María Ángeles
A1 Balsinde Rodríguez, Jesús
K1 Arachidonic acid
K1 Ácido araquidónico
K1 Eicosanoids
K1 Eicosanoides
K1 Phospholipases
K1 Fosfolipasas
K1 Monocytes
K1 Monocitos
K1 Macrophages
K1 Macrófagos
AB Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis. AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens. In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108. All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process. Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process. Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect. These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
PB MDPI
SN 2073-4409
YR 2019
FD 2019
LK https://uvadoc.uva.es/handle/10324/55855
UL https://uvadoc.uva.es/handle/10324/55855
LA eng
NO Cells, 2019, vol. 8, n. 8, 799
NO Producción Científica
DS UVaDOC
RD 01-may-2024