RT info:eu-repo/semantics/article T1 Assessment of the effect of nitrogen concentration on fermentation and selection of a highly competitive Saccharomyces cerevisiae strain for efficient ethanol production A1 Portero Barahona, Patricia A1 Martín Gil, Jesús A1 Martín Ramos, Pablo A1 Briones Pérez, Ana A1 Carvajal Barriga, Enrique Javier K1 Ethanol K1 Etanol K1 Fermentation K1 Fermentación K1 Nitrogen K1 Nitrógeno AB The optimum nitrogen concentration for media supplementation and strain dominance are aspects of key importance to the industrial production of ethanol with a view to reducing costs and increasing yields. In this work, these two factors were investigated for four ethanologenic Saccharomyces cerevisiae strains (CLQCA-INT-001, CLQCA-INT-005, CLQCA-10-099, and UCLM 325), selected from the screening of 150 isolates, mostly from Ecuadorian yeast biodiversity. The effect of nitrogen concentration was assessed in terms of cellular growth, glucose consumption and ethanol production, and the yeast strains’ dominance was evaluated in continuous co-fermentation with cellular recycling by mitochondrial DNA analyses. Among the four selected yeast strains under study, CLQCA-INT-005 presented the highest glucose consumption at a nitrogen supplement concentration as low as 0.4 g·L−1, attaining an ethanol yield of up to 96.72% in 24 h. The same yeast strain was found to be highly competitive, showing a dominance of 80% after four cycles of fermentation in co-culture. Thus, CLQCA-INT-005 may be deemed as a very promising candidate to be used both at pilot-plant scale and at industrial scale cellulosic ethanol production. PB MDPI SN 1996-1073 YR 2019 FD 2019 LK https://uvadoc.uva.es/handle/10324/55877 UL https://uvadoc.uva.es/handle/10324/55877 LA eng NO Energies, 2019, vol. 12, n. 13, 2614 NO Producción Científica DS UVaDOC RD 22-nov-2024