RT info:eu-repo/semantics/article
T1 Sequestration of 9-Hydroxystearic acid in FAHFA (fatty acid esters of hydroxy fatty acids) as a protective mechanism for colon carcinoma cells to avoid apoptotic cell death
A1 Rodríguez, Juan Pablo
A1 Guijas, Carlos
A1 Astudillo del Valle, Alma María
A1 Rubio, Julio M.
A1 Balboa García, María Ángeles
A1 Balsinde Rodríguez, Jesús
K1 Colorectal cancer
K1 Cáncer colorrectal
K1 Hydroxystearic acid
K1 Ácido hidroxiesteárico
K1 Apoptosis
AB Hydroxy fatty acids are known to cause cell cycle arrest and apoptosis. The best studied of them, 9-hydroxystearic acid (9-HSA), induces apoptosis in cell lines by acting through mechanisms involving different targets. Using mass spectrometry-based lipidomic approaches, we show in this study that 9-HSA levels in human colorectal tumors are diminished when compared with normal adjacent tissue. Since this decrease could be compatible with an escape mechanism of tumors from 9-HSA-induced apoptosis, we investigated different features of the utilization of this hydroxyfatty acid in colon. We show that in colorectal tumors and related cell lines such as HT-29 and HCT-116, 9-HSA is the only hydroxyfatty acid constituent of branched fatty acid esters of hydroxyfatty acids (FAHFA), a novel family of lipids with anti-inflammatory properties. Importantly, FAHFA levels in tumors are elevated compared with normal tissue and, unlike 9-HSA, they do not induce apoptosis of colorectal cell lines over a wide range of concentrations. Further, the addition of 9-HSA to colon cancer cell lines augments the synthesis of different FAHFA before the cells commit to apoptosis, suggesting that FAHFA formation may function as a buffer system that sequesters the hydroxyacid into an inactive form, thereby restricting apoptosis.
PB MDPI
SN 2072-6694
YR 2019
FD 2019
LK https://uvadoc.uva.es/handle/10324/55994
UL https://uvadoc.uva.es/handle/10324/55994
LA eng
NO Cancers, 2019, vol. 11, n. 4, 524
NO Producción Científica
DS UVaDOC
RD 27-dic-2024