RT info:eu-repo/semantics/article
T1 Chiral and achiral separation of ten flavanones using supercritical fluid chromatography. Application to bee pollen analysis
A1 Ares Sacristán, Ana María
A1 Bernal del Nozal, José
A1 Janvier, Andrea
A1 Toribio Recio, Laura
K1 Flavanones
K1 Flavanonas
K1 Bee pollen
K1 Polen de abeja
AB The separation of ten flavanones (flavanone, 2′-hydroxyflavanone, 4’-hydroxyflavanone, 6-hydroxyflavanone, 7-hydroxyflavanone, naringenin, naringin, hesperetin, pinostrobin, and taxifolin) using supercritical fluid chromatography and considering achiral and chiral approaches has been studied in this work. For this purpose, different chiral stationary phases and organic modifiers have been checked. Considering the achiral separation, the best results were obtained with the Lichrospher 100 Diol column at 35°C, 3mL/min, 150 bar and a gradient of 2-propanol from 5% to 50%. The baseline separation of the ten compounds was achieved in 18 minutes. Using the chiral column Chiralpak AD, the separation of the ten pairs of enantiomers was obtained in 32 minutes. In this case, the chromatographic conditions were 30°C, 3mL/min, 150 bar and the organic modifier was a mixture ethanol/methanol (80:20) containing 0.1% of trifluoroacetic acid applied in an elution gradient from 15% to 50%. The applicability of the proposed chiral method was assessed by analysing bee pollen samples and 2S-pinostrobin was determined in some of them.
PB Elsevier
SN 0021-9673
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/56793
UL https://uvadoc.uva.es/handle/10324/56793
LA eng
NO Journal of Chromatography A, 2022, vol. 1685, 463633
NO Producción Científica
DS UVaDOC
RD 26-abr-2024