RT info:eu-repo/semantics/article
T1 Protease‐sensitive, VEGF‐mimetic peptide, and IKVAV laminin‐derived peptide sequences within elastin‐like recombinamer scaffolds provide spatiotemporally synchronized guidance of angiogenesis and neurogenesis
A1 González Pérez, Fernando
A1 Alonso Rodrigo, Matilde
A1 González de Torre, Israel
A1 Santos García, María Mercedes
A1 Rodríguez Cabello, José Carlos
K1 Elastin-like recombinamers
K1 Hydrogels
K1 Innervation
K1 Tunable proteolytic sequences
K1 Vascularization
K1 32 Ciencias Médicas
K1 23 Química
AB Spatiotemporal control of vascularization and innervation is a desiredhallmark in advanced tissue regeneration. For this purpose, we design a 3Dmodel scaffold, based on elastin-like recombinamer (ELR) hydrogels. Thiscontains two interior and well-defined areas, small cylinders, withdifferentiated bioactivities with respect to the bulk. Both are constructed on aprotease sensitive ELR with a fast-proteolyzed domain, but one bears aVEGF-mimetic peptide (QK) and the other a laminin-derived pentapeptide(IKVAV), to promote angiogenesis and neurogenesis, respectively. The outerbulk is based on a slow proteolytic sequence and RGD cell adhesion domains.In vitro studies show the effect of QK and IKVAV peptides on the promotion ofendothelial cell and axon spreading, respectively. The subcutaneousimplantation of the final 3D scaffold demonstrates the ability tospatiotemporally control angiogenesis and neurogenesis in vivo. Specifically,the inner small cylinder containing the QK peptide promotes fastendothelialization, whereas the one with IKVAV peptide promotes fastneurogenesis. Both, vascularization and innervation take place in advance ofthe bulk scaffold infiltration. This scaffold shows that it is possible to inducevascularization and innervation in predetermined areas of the scaffold wellahead to the bulk infiltration. That significantly increases the efficiency of theregenerative activity.
PB Wiley
SN 2192-2640
YR 2022
FD 2022
LK https://uvadoc.uva.es/handle/10324/57154
UL https://uvadoc.uva.es/handle/10324/57154
LA eng
NO Advanced Healthcare Materials, 2022.Volume11, Issue 22, 2201646
NO Producción Científica
DS UVaDOC
RD 06-ago-2024