RT info:eu-repo/semantics/article
T1 Effect of different broad waveband lights on membrane lipids of a cyanobacterium, synechococcus sp., as determined by UPLC-QToF-MS and vibrational spectroscopy
A1 Montero, Olimpio
A1 Velasco, Marta
A1 Sanz Arranz, Aurelio
A1 Rull Pérez, Fernando
K1 Synechococcus sp.
K1 UPLC-MS
K1 Vibrational spectroscopy
K1 Raman
K1 Lipids
K1 Pigments
K1 22 Física
AB Differential profile of membrane lipids and pigments of a Synechococcus sp. cyanobacterial strain cells exposed to blue, green, red and white light are determined by means of liquid chromatography and mass spectrometry or diode array detection. Raman and ATR-IR spectra of intact cells under the diverse light wavebands are also reported. Blue light cells exhibited an increased content of photosynthetic pigments as well as specific species of membrane glycerolipids as compared to cells exposed to other wavebands. The A630/A680 ratio indicated an increased content of phycobilisomes (PBS) in the blue light-exposed cells. Some differences in the protein conformation between the four light waveband-exposed cells were deduced from the variable absorbance at specific wavenumbers in the FT-Raman and ATR-FTIR spectra, in particular bands assigned to amide I and amide II. Bands from 1180 to 950 cm−1 in the ATR-FTIR spectrum suggest degraded outer membrane polysaccharide in the blue light-exposed cells.
PB MDPI
YR 2016
FD 2016
LK https://uvadoc.uva.es/handle/10324/57283
UL https://uvadoc.uva.es/handle/10324/57283
LA eng
NO Biology, 2016, vol. 5, n. 2, p. 22
NO Producción Científica
DS UVaDOC
RD 18-dic-2024