RT info:eu-repo/semantics/article T1 Waste-to-fuel technologies for the bioconversion of carrot discards into biobutanol A1 López Linares, Juan Carlos A1 Coca Sanz, Mónica A1 Plaza Lazaro, Pedro Enrique A1 Lucas Yagüe, Susana A1 García Cubero, María Teresa K1 Hydrothermal pretreatment K1 Pretratamiento hidrotérmico K1 Enzymatic hydrolysis K1 Hidrólisis enzimática K1 Biofuels K1 Biocarburantes K1 Biobutanol AB Carrot discard was evaluated as a raw material for acetone-butanol-ethanol (ABE) fermentation. Different strategies based on hydrothermal pretreatment and/or enzymatic hydrolysis were compared for biobutanol production from carrot discard pulp. In addition, the use of different types of enzymes and diverse enzyme mixtures were evaluated. In this way, total sugar recoveries of up to 76%, and butanol and ABE concentrations of 7.4 and 11.5 g/L, respectively (74 g butanol and 115 g ABE/kg carrot pulp), were achieved when the carrot discard pulp was enzymatically hydrolyzed, without pretreatment, using a mixture of enzymes of Cellic CTec2 and Viscozyme L at a dosage of 0.1 and 0.2 g/g, respectively. When a hydrothermal pretreatment was applied, a total sugar recovery of 88%, 6.9 g/L butanol and 10.1 g/L ABE (69 g butanol and 101 g ABE/kg carrot pulp) were attained using the same mixture of enzymes. In this way, no hydrothermal pretreatment would be necessary to produce ABE from carrot discard, which is very interesting for the profitability of the process. Furthermore, the carrot discard juice yielded 6.4 and 9 g/L butanol and ABE, respectively, showing that all the carrot discards could be used for ABE production. PB Elsevier SN 0960-1481 YR 2022 FD 2022 LK https://uvadoc.uva.es/handle/10324/57407 UL https://uvadoc.uva.es/handle/10324/57407 LA eng NO Renewable Energy, 2023, Volume 202, Pages 362-369 NO Producción Científica DS UVaDOC RD 24-nov-2024