RT info:eu-repo/semantics/article
T1 New trends in quantitative assessment of the corneal barrier function
A1 Guimerà Brunet, Anton
A1 Illa, Xavi
A1 Traver, Estefania
A1 Herrero, Carmen
A1 Maldonado López, Miguel José
A1 Villa, Rosa
K1 Electrical impedance spectroscopy
K1 Corneal epithelium
K1 Permeability
K1 Barrier function
K1 33 Ciencias Tecnológicas
K1 32 Ciencias Médicas
AB The cornea is a very particular tissue due to its transparency and its barrier function as it has to resist against the daily insults of the external environment. In addition, maintenance of this barrier function is of crucial importance to ensure a correct corneal homeostasis. Here, the corneal epithelial permeability has been assessed in vivo by means of non-invasive tetrapolar impedance measurements, taking advantage of the huge impact of the ion fluxes in the passive electrical properties of living tissues. This has been possible by using a flexible sensor based in SU-8 photoresist. In this work, a further analysis focused on the validation of the presented sensor is performed by monitoring the healing process of corneas that were previously wounded. The obtained impedance measurements have been compared with the damaged area observed in corneal fluorescein staining images. The successful results confirm the feasibility of this novel method, as it represents a more sensitive in vivo and non-invasive test to assess low alterations of the epithelial permeability. Then, it could be used as an excellent complement to the fluorescein staining image evaluation.
PB MDPI
SN 1424-8220
YR 2014
FD 2014
LK https://uvadoc.uva.es/handle/10324/57504
UL https://uvadoc.uva.es/handle/10324/57504
LA eng
NO Sensors, 2014, vol. 14, n. 5, p. 8718-8727
NO Producción Científica
DS UVaDOC
RD 18-nov-2024