RT info:eu-repo/semantics/article
T1 Amperometric biosensor for oxalate determination in urine using sequential injection analysis
A1 Rodríguez, José Antonio
A1 Hernández, Prisciliano
A1 Salazar Pereda, Verónica
A1 Castrillejo Hernández, María Yolanda
A1 Barrado Esteban, Enrique
K1 Oxalate
K1 Urine
K1 Sequential injection analysis
K1 Magnetic particles
K1 23 Química
K1 2301 Química Analítica
AB An amperometric flow biosensor for oxalate determination in urine samples after enzymatic reaction with oxalate oxidase immobilized on a modified magnetic solid is described. The solid was magnetically retained on the electrode surface of an electrode modified with Fe (III)-tris-(2-thiopyridone) borate placed into a sequential injection system preceding the amperometric detector. The variables involved in the system such as flow rate, aspired volumes (modified magnetic suspension and sample) and reaction coil length were evaluated using a Taguchi parameter design. Under optimal conditions, the calibration curve of oxalate was linear between 3.0–50.0 mg·L−1, with a limit of detection of 1.0 mg·L−1. The repeatability for a 30.0 mg·L−1 oxalate solution was 0.7%. The method was validated by comparing the obtained results to those provided by the spectrophotometric method; no significant differences were observed.
PB MDPI
SN 1420-3049
YR 2012
FD 2012
LK https://uvadoc.uva.es/handle/10324/57788
UL https://uvadoc.uva.es/handle/10324/57788
LA eng
NO Molecules, 2012, vol. 17, n. 8, p. 8859-8871
NO Producción Científica
DS UVaDOC
RD 07-ago-2024