RT info:eu-repo/semantics/article
T1 Embryonic Cerebrospinal Fluid Collaborates With the Isthmic Organizer To Regulate Mesencephalic Gene Expression
A1 Parada, Carolina
A1 Martín, Cristina
A1 Alonso Revuelta, María Isabel
A1 Moro Balbás, José Antonio
A1 Bueno i Torrens, David
A1 Gato Casado, Ángel Luis
K1 Líquidos orgánicos
K1 Neurología
K1 Cerebro
K1 Embriología humana
AB Early in development, the behavior of neuroepithelialcells is controlled by several factors acting in a developmentallyregulated manner. Recently it has beenshown that diffusible factors contained within embryoniccerebrospinal fluid (CSF) promote neuroepithelialcell survival, proliferation, and neurogenesis in mesencephalicexplants lacking any known organizing center.In this paper, we show that mesencephalic and mesencephalicþ isthmic organizer explants cultured only withbasal medium do not express the typically expressedmesencephalic or isthmic organizer genes analyzed(otx2 and fgf8, respectively) and that mesencephalicexplants cultured with embryonic CSF-supplementedmedium do effect such expression, although theyexhibit an altered pattern of gene expression, includingectopic shh expression domains. Other trophic sourcesthat are able to maintain normal neuroepithelial cellbehavior, i.e., fibroblast growth factor-2, fail to activatethis ectopic shh expression. Conversely, the expressionpattern of the analyzed genes in mesencephalic þ isthmicorganizer explants cultured with embryonic cerebrospinalfluid-supplemented medium mimics thepattern for control embryos developed in ovo. We demonstratethat embryonic CSF collaborates with the isthmicorganizer in regulation of the expression pattern ofsome characteristic neuroectodermal genes duringearly stages of central nervous system (CNS) development,and we suggest that this collaboration is notrestricted to the maintenance of neuroepithelial cellsurvival. Data reported in this paper corroborate thehypothesis that factors contained within embryonicCSF contribute to the patterning of the CNS duringearly embryonic development.
PB Willey InterScience
SN 0360-4012
YR 2005
FD 2005
LK http://uvadoc.uva.es/handle/10324/5795
UL http://uvadoc.uva.es/handle/10324/5795
LA eng
NO Journal of Neuroscience Research, 2005, vol. 82. p.333-345
NO Producción Científica
DS UVaDOC
RD 22-nov-2024