RT info:eu-repo/semantics/article
T1 Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography
A1 Ares Sacristán, Ana María
A1 Toribio Recio, Laura
A1 García Villalba, Rocío
A1 Villalgordo, Jose M.
A1 Althobaiti, Yusuf
A1 Tomás Barberán, Francisco A.
A1 Bernal del Nozal, José
K1 Química analítica
K1 High-performance liquid chromatography
K1 Gut microbiota metabolites
K1 Urolithins
K1 Cromatografía líquida de alta resolución
K1 Metabolitos de la microbiota intestinal
K1 Urolitinas
K1 2301 Química Analítica
AB Urolithins are gut microbiota metabolites produced in humans after consuming foods containing ellagitannins and ellagic acid. Three urolithin metabotypes have been reported for different individuals depending on the final urolithins produced. After absorption, they are conjugated with glucuronic acid (phase II metabolism), and these are the main circulating metabolites in plasma and reach different tissues. Different regioisomeric isomers of urolithin glucuronides have been described. Still, their identification and quantification in humans have not been properly reported due to resolution limitations in their analysis by reversed-phase high-performance liquid chromatography. In the present study, we report a novel method for separating these isomers using supercritical fluid chromatography. With this method, urolithin A 3- and 8-glucuronide, isourolithin A 3- and 9- glucuronide, and urolithin B 3-glucuronide (8-hydroxy urolithin 3-glucuronide; 3-hydroxy urolithin 8-glucuronide; 3-hydroxyurolithin 9-glucuronide; 9-hydroxyurolithin 3-glucuronide; and urolithin 3-glucuronide) were separated in less than 15 min. The proposed method was applied to successfully analyze these metabolites in urine samples from different volunteers belonging to different metabotypes.
PB American Chemical Society
SN 0021-8561
YR 2023
FD 2023
LK https://uvadoc.uva.es/handle/10324/58690
UL https://uvadoc.uva.es/handle/10324/58690
LA eng
NO Journal of Agricultural and Food Chemistry, 2023, vol. 71, n. 6. p. 3033–3039.
NO Producción Científica
DS UVaDOC
RD 22-nov-2024