RT info:eu-repo/semantics/article T1 Integrated ozonation-enzymatic hydrolysis pretreatment of sugarcane bagasse: enhancement of sugars released to expended ozone ratio A1 Osuna Laveaga, Daryl Rafael A1 García Depraect, Octavio A1 Vallejo Rodríguez, Ramiro A1 López López, Alberto A1 León Becerril, Elizabeth K1 Biomass energy K1 Lignocellulosic biomass K1 Renewable and Green Energy K1 Renewable energy resources K1 Energías renovables K1 Hidrólisis K1 Ozone K1 Particle size determination K1 Sugarcane K1 Caña de azúcar K1 Moisture content K1 Sugar yield K1 3103.08 Gestión de la Producción Vegetal AB The combined effects of three key ozonation process parameters on the integrated ozonation-enzymatic hydrolysis pretreatment of sugarcane bagasse (SCB) were investigated, with emphasis on the relationship between sugar release and ozone consumption. A lab-scale fixed bed reactor was employed for ozonation at varying ozone doses (50, 75 and 100 mg O3/g SCB), particle sizes (420, 710 and 1000 µm) and moisture contents (30, 45 and 60% w/w) in multifactorial experiments, keeping a residence time of 30 min. The ozonated SCB showed a reduction in the content of acid-insoluble lignin from 26.6 down to 19.1% w/w, while those of cellulose and hemicellulose were retained above 45.5 and 13.6% w/w, with recoveries of 100–89.9 and 83.5–72.7%, respectively. Ozone-assisted enzymatic hydrolysis allowed attaining glucose and xylose yields as high as 45.0 and 37.8%, respectively. The sugars released/ozone expended ratio ranged between 2.3 and 5.7 g sugars/g O3, being the higher value achieved with an applied ozone input of 50 mg O3/g SCB and SCB with 420 µm particle size and 60% moisture. Such operating conditions led to efficient ozone utilization (<2% unreacted ozone) with a yield of 0.29 g sugars/g SCB. Overall, the amount of sugars released relative to the ozone consumed was improved, entailing an estimated cost of ozonation of USD 34.7/ton of SCB, which could enhance the profitability of the process. PB MDPI SN 2227-9717 YR 2020 FD 2020 LK https://uvadoc.uva.es/handle/10324/59045 UL https://uvadoc.uva.es/handle/10324/59045 LA eng NO Processes, 2020, Vol. 8, Nº. 10, 1274 NO Producción Científica DS UVaDOC RD 24-nov-2024