RT info:eu-repo/semantics/article
T1 Dark fermentation process response to the use of undiluted tequila vinasse without nutrient supplementation
A1 Rodríguez Reyes, Juan José
A1 García Depraect, Octavio
A1 Castro Muñoz, Roberto
A1 León Becerril, Elizabeth
K1 Procesos químicos
K1 Renewable and Green Energy
K1 Bioenergy
K1 Dark fermentation
K1 Tequila vinasse
K1 Bio-hydrogen
K1 Bioenergía
K1 Fermentación oscura
K1 Vinaza de tequila
K1 Biohidrógeno
K1 3303.03 Procesos Químicos
AB The technical feasibility of valorizing tequila vinasse (TV), a wastewater with high pollution potential, through the production of biogenic hydrogen via dark fermentation, has long been proven in diverse lab-scale reactors that were operated either in batch or continuous mode. However, such systems have mainly been tested with diluted streams and nutrient supplementation, hindering the techno-economic attractiveness of the TV-to-hydrogen concept at large scale. In this study, the feasibility of producing hydrogen from high-strength undiluted TV with no added extra nutrients was evaluated under batch mesophilic conditions. Additionally, the use of two different acidogenic inocula obtained either by heat or heat-aeration pretreatment was investigated to get a greater understanding of the effect of inoculum type on the process. The results obtained showed that the TV utilized herein contained macro- and micro-nutrients high enough to support the hydrogenogenic activity of both cultures, entailing average hydrogen yields of 2.4–2.6 NL H2/L vinasse and maximum hydrogen production rates of 1.4–1.9 NL H2/L-d. Interestingly, the consumption of lactate and acetate with the concomitant production of butyrate was observed as the main hydrogen-producing route regardless of the inoculum, pointing out the relevance of the lactate-driven dark fermentative process. Clostridium beijerinckii was ascertained as key bacteria, but only in association with microorganisms belonging to the genera Enterobacter and Klebsiella, as revealed by phylogenetic analyses.
PB MDPI
YR 2021
FD 2021
LK https://uvadoc.uva.es/handle/10324/59320
UL https://uvadoc.uva.es/handle/10324/59320
LA eng
NO Sustainability, 2021, vol. 13, n. 19, 11034
NO Producción Científica
DS UVaDOC
RD 24-nov-2024